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High Sensitivity Mouse IFN-γ (Interferon Gamma) ELISA Kit (E-HSEL-M0007)

All Size Price Qty
96T $ 520.00
48T $ 416.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary

New series of Elabscience® High Sensitivity (HS) ELISA kits have been iteratively upgraded through R&D process to increase the sensitivity of the traditional ELISA kits by 2-10 times, and can detect the protein concentration of fg level. High Sensitivity (HS) ELISA Kit focuses on the detection of cytokines, and this series can effectively solve the problem of low detection rate when detecting low concentration samples such as serum and plasma.

Sensitivity 0.84 pg/mL
Detection Range 1.56-100 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Mouse
Specificity This kit recognizes Mouse IFN-γ in samples.No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Uniport ID P01580
Research Area Cancer , Immunology , Stem Cells
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