High Sensitivity Mouse IL-2 (Interleukin 2) ELISA Kit (E-HSEL-M0011)
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For research use only.
Product Summary
The new series of Elabscience® High Sensitivity (HS) ELISA kits have been iteratively upgraded through R&D process to increase the sensitivity of the traditional ELISA kits by 2-10 times, and can detect the protein concentration of fg level. High Sensitivity (HS) ELISA Kit focuses on the detection of cytokines, and this series can effectively solve the problem of low detection rate when detecting low concentration samples such as serum and plasma.
| Sensitivity | 0.94 pg/mL |
| Detection Range | 1.56-100 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Mouse |
| Specificity | This kit recognizes Mouse IL-2 in samples.No significant cross-reactivity or interference between and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum,plasma, urine and tissue homogenate |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Mouse IL-2 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Mouse IL-2 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Mouse IL-2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 5 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.
Background
Interleukin 2 (IL-2), also known as T cell growth factor (TCGF),produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance.Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance.In vivo,IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 have shown an important role for IL-2 in T cell activation and expansion.It can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells.
| Gene Alias | IL-2 |
| Gene ID | 16183 |
| Uniport ID | P04351 |
| Protein Alias | IL-2 |
| Research Area | Immunology |
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Videos
Resources
• High Sensitivity ELISA for IL-6 Detection and Reliable Quantification of Inflammatory Biomarkers in Research
• High Sensitivity vs Traditional ELISA: Improved Cytokine Detection and Accuracy
• ELISA Explained: How It Works and How to Interpret Results with Standard Curve Analysis
• How ELISA Microplates Affect Experimental Results in ELISA Assays
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