Ultra Sensitive
High Sensitivity Rat IL-1β (Interleukin 1 Beta) ELISA Kit (E-HSEL-R0002)
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For research use only.
Product Summary
New series of Elabscience® High Sensitivity (HS) ELISA kits have been iteratively upgraded through R&D process to increase the sensitivity of the traditional ELISA kits by 2-10 times, and can detect the protein concentration of fg level. High Sensitivity (HS) ELISA Kit focuses on the detection of cytokines, and this series can effectively solve the problem of low detection rate when detecting low concentration samples such as serum and plasma.
| Sensitivity | 0.2 pg/mL |
| Detection Range | 0.31-20 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Rat |
| Specificity | This kit recognizes Rat IL-1β in samples.No significant cross-reactivity or interference between Rat IL-1β and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Rat IL-1β were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Rat IL-1β were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Rat IL-1β was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Rat IL-1β and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve.
Background
| Uniport ID | Q63264 |
| Research Area | Cancer , Cardiovascular , Immunology , Metabolism |
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Videos
Resources
• High Sensitivity ELISA for IL-6 Detection and Reliable Quantification of Inflammatory Biomarkers in Research
• High Sensitivity vs Traditional ELISA: Improved Cytokine Detection and Accuracy
• Tips for ELISA Failure
• ELISA Explained: How It Works and How to Interpret Results with Standard Curve Analysis
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