Cell Function
Cell Function FAQs
In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.
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JC-1 detects the change of mitochondrial membrane potential which happens during the early stage of apoptosis, TUNEL reflects the situation of DNA fragmentation during the late stage of apoptosis. A JC-1 positive but TUNEL negative result shows that the cells were in the early stage of apoptosis.
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The equilibrition process is to make a better working condition of TdT enzyme that benefit a better staining result.
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The best linearity of CCK-8 is between 0.2-2.5 (Optical Density).
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Yes it is. But Triton X-100 has more acurate effect, 0.2% Triton X-100 and 3% BSA in PBS could be used to protect cells.
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Normally, it is better to detect in 1-2 hours after staining. If the cells required further incubation, it can be kept in CO2 incubator with no more than 48 hours. When using microscope, it is recommanded to turn the illumination down and extend exposure time.
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It is better to start with the lowest illumination and lowest exposure time, then gradually increase the exposure time and fluorescence brightness. Over time exposure can easily cause dye bleaching and overexposure. It is better to keep the negative control without background fluorescence and make the positive control with well resolution. The exposure conditions for the same fluorescein in different groups need to be consistent.
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It is possible. But it have to be notice that the reagents must fully cover the sample and keep moist. It may require more reagent than the recommanded amount. The result should be observed by inverted microscope and cannot be sealed.
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Half dose reagent can be used with no more than 5*10^5 cells following the two-step operation.
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BSA is used for protecting cells from mechanical damage and losing during centrifuge and washing, especially for suspension cells.
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Yes, it is. The sample can be fixed with 2-4% paraformeldehyde for 30-60 minutes in room temperature, then kept with PBS in 4 ℃. The sample can be stored for no more than 3 days.
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No. Mito-Tracker Green is a green fluorescent probe specifically designed for labeling mitochondria in live cells. Unlike JC-1, its staining mechanism is independent of mitochondrial membrane potential.
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Yes, Annexin V kit can be used with fluorescence microscopy to observe apoptosis in situ. However, apoptotic cells often exhibit reduced adhesion (e.g., poor attachment or floating), which may result in detection failure during observation.
