Hello, I would like to ask if there is a recommended Annexin V kit for cells treated with doxorubicin.

Doxorubicin can interfere with the PE, PE-TR, and PerCP/Cy5.5 channels. If the flow cytometer has a 405 nm excitation laser, it is recommended to use E-CK-A258 Annexin V-APC/DAPI Apoptosis Kit.

Does this DMF evaporate?

Yes, DMF is quite volatile, so after use, make sure to tighten the cap and seal it with parafilm..

For the Annexin V kit, the nuclear dye is PI, but the flow cytometer only has PerCP/Cy5.5 and PE channels. Which one would be more suitable?

Using PE would result in higher fluorescence intensity, but using PerCP/Cy5.5 would reduce interference from FITC.

For paraffin sections that have already been labeled with TUNEL and show positive signals, is there a way to remove the counterstaining, or is the only option to replace the section and start over?

There is no way to remove the counterstaining; the only option is to replace the section and redo it. The principle of TUNEL is to label fluorescently labeled dUTP deoxyribonucleotides onto the 3'-OH ends of fragmented DNA. The 3' ends of apoptotic cells that have already undergone TUNEL may already be occupied, so repeating the procedure would directly affect the experimental results.

Does E-CK-A383 target both Caspase-3 and Caspase-7?

Since activated Caspase-3 and Caspase-7 can both recognize and cleave the same DEVD sequence, they are generally detected together.

Could you please confirm whether E-CK-A383 targets both Caspase-3 and Caspase-7? The manual seems to emphasize Caspase-3, so I wanted to double-check.

Since both activated Caspase-3 and Caspase-7 can recognize and cleave the same DEVD substrate sequence, they are typically detected together.

The cell sample is infected with a virus carrying a GFP tag. Will the detection wavelength of CCK8 be affected?

It will not be affected, as CCK8 measures absorbance while GFP measures fluorescence.

What is the molecular weight of this PI?

CAS number of PI is 25535-16-4, molecular formula is C27H34I2N4, molecular weight is 668.4 Da.

Cell Function

Cell Function FAQs

In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.

Doxorubicin can interfere with the PE, PE-TR, and PerCP/Cy5.5 channels. If the flow cytometer has a 405 nm excitation laser, it is recommended to use E-CK-A258 Annexin V-APC/DAPI Apoptosis Kit.
Yes, DMF is quite volatile, so after use, make sure to tighten the cap and seal it with parafilm..
Using PE would result in higher fluorescence intensity, but using PerCP/Cy5.5 would reduce interference from FITC.
There is no way to remove the counterstaining; the only option is to replace the section and redo it. The principle of TUNEL is to label fluorescently labeled dUTP deoxyribonucleotides onto the 3'-OH ends of fragmented DNA. The 3' ends of apoptotic cells that have already undergone TUNEL may already be occupied, so repeating the procedure would directly affect the experimental results.
Since activated Caspase-3 and Caspase-7 can both recognize and cleave the same DEVD sequence, they are generally detected together.
Since both activated Caspase-3 and Caspase-7 can recognize and cleave the same DEVD substrate sequence, they are typically detected together.
It will not be affected, as CCK8 measures absorbance while GFP measures fluorescence.
CAS number of PI is 25535-16-4, molecular formula is C27H34I2N4, molecular weight is 668.4 Da.

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Cell Function

Cell Function

Cell Function FAQs

Q1. Hello, I would like to ask if there is a recommended Annexin V kit for cells treated with doxorubicin.

Q2. Does this DMF evaporate?

Q3. For the Annexin V kit, the nuclear dye is PI, but the flow cytometer only has PerCP/Cy5.5 and PE channels. Which one would be more suitable?

Q4. For paraffin sections that have already been labeled with TUNEL and show positive signals, is there a way to remove the counterstaining, or is the only option to replace the section and start over?

Q5. Does E-CK-A383 target both Caspase-3 and Caspase-7?

Q6. Could you please confirm whether E-CK-A383 targets both Caspase-3 and Caspase-7? The manual seems to emphasize Caspase-3, so I wanted to double-check.

Q7. The cell sample is infected with a virus carrying a GFP tag. Will the detection wavelength of CCK8 be affected?

Q8. What is the molecular weight of this PI?