Cell Function
Cell Function FAQs
In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.
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Plant tissues have cell walls and chloroplasts, which may interfere with JC-1 detection. We have not validated this application and cannot confirm its effectiveness. If testing, we recommend consulting relevant literature and conducting preliminary experiments.
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One assay provides 1 mL BMDC differentiation medium (for 24-well plate, 1 mL/well) and 0.5 mL BMDC maturation medium. 200 assays can culture ~1×10^7 mouse bone marrow cells. After 7 days of differentiation, expect 1–3×10^7 immature DCs (70–80% purity). Following 24-hour maturation, yield 1–3×10^7 mature DCs (70–80% purity).
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For optimal sensitivity: Use ≥1×10^6 cells or ≥50 mg tissue per experiment to achieve a protein concentration of 1–4 mg/mL. Lower amounts may reduce accuracy due to insufficient active caspase 3/7 (OD values below the linear detection range of 0.05–0.6).
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The copper ions in the EdU kit may quench GFP/RFP/mCherry fluorescence. If you do NOT require GFP detection, the kit will not impact EdU assay results.
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EDTA-containing trypsin can be used, but ensure thorough washing with PBS after digestion to remove residual EDTA.
