Cell Function
Cell Function FAQs
In order to provide better customer support and answer customers' questions about cell function assay products, we have compiled and published answers to the frequently-asked questions (FAQs) about cell function assay products, and will continue to update them.
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No. Elab Fluor® 594-11-dUTP is a single component, while Labeling Solution is a multi-component mixed reagent that includes labeled dUTP. Therefore, it is recommended that customers purchase TUNEL kits directly for experiments.
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① The test sample does not contain fluorescence, and all TUNEL kits can be used. ② If the experimental sample has red light, it is recommended to use One-step TUNEL Assay Kit (Green, FITC) item No. : E-CK-A320 or One-step TUNEL Assay Kit (Green, Elab Fluor®488) item No. : E-CK-A320.
In general, the anti-quenching effect of Elab Fluor®488 is better than that of FITC, and One-step TUNEL Assay Kit (Green, Elab Fluor®488) is recommended if the green fluorescence of a sample is to be observed over a long period of time.
③ If the experimental sample has green light, One-step TUNEL Assay Kit (Red, Elab Fluor®594) is recommended: E-CK-A322 or One-step TUNEL Assay Kit (Red, Elab Fluor®555) Item No. E-CK-A325. The Elab Fluor®594 Assay Kit (Red, Elab Fluor®594), item No. E-CK-A322, is preferred if the sample has a strong green fluorescence.
If both Red and green fluorescence are present in the test sample, One-step TUNEL Assay Kit (Red, Elab Fluor®647) is recommended: E-CK-A324, but Elab Fluor®647 has higher requirements for fluorescence microscopy, requiring the microscope to be equipped with Cy5 or Elab Fluor®647 filters, which are generally equipped with confocal microscopes. -
It is recommended to incubate 500μL of Labeling Working Solution per well.
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The negative control group needs to perform labeling protocol too. However, it should be noted that the Labeling Working Solution in the negative control group are different from the other positive control or experimental group.
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It requires DAPI working Solution to cover the sample.
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It can be washed and soaked in PBS and stored in a refrigerator at 4°C.
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Yes, the frozen samples of -80°C can be made into frozen slices, and then the frozen slices can be used for tunel detection.
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Generally, the thickness of frozen slices is 8-10 µm. If the slice is thick, the incubation time of protease K can be extended appropriately according to the actual situation.
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The process of making paraffin sections requires baking sheets, but the process of tunel dyeing after the section is made does not require baking sheets.
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PBS added during washing steps must completely cover the sample, and ensure the sample moist during the experiment to prevent the failure of the experiment caused by dry slides.
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It is recommended to incubate at room temperature, about 25℃. The AnnexinV staining process is actually the process of binding the AnnexinV protein specifically to phosphatidylserine on the cell membrane, and this process is appropriate when the cell function and enzyme activity are normal.
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According to the specifications of the kit and the method of use, the specific number of samples can be determined. There are two ways to use annexin V in the instructions, one step method and two step method. The one-step annexin V dosage is 5μL, the two-step annexin V dosage is 2.5μL, and we are provided in accordance with the one-step method (maximum dosage), which means that if you do it in two steps, the kit size of 20 Assays can do 40 samples, and so on.
