Cell Types

T/B/NK Cell Population Detection

Detection of T/B/NK (4-color) in human peripheral blood
Detection of T/B/NK (4-color) in C57BL/6 mouse peripheral blood
Detection of TB cells (4-color) in mouse lymph node
Detection of T/B/NK (5-color) in Rat Spleen
Detection of T/B/NK (3-color) in Rat Spleen
CD4+/CD8+T Cell Population Detection

Detection of T cells (4-color) in Human Peripheral Blood
Detection of T Cells (2-color) in Mouse Thymus
Detection of Lymphocyte (4 colors) in Mouse Tumor
Treg Cell Population Detection

Detection of Treg (3-color) in Mouse Spleen
Detection of Treg (6-color) in Human Peripheral Blood
Th1/Th2/ Th17 Cell Population Detection

Detection of Th1/Th2 (4-color) in Human Peripheral Blood PBMC
Detection of Th17 (3-color) in Human Peripheral Blood PBMC

Current Position: Home>>Cell Types>>T/B/NK Cell Population Detection>>Detection of T/B/NK (5-color) in Rat Spleen

Detection of T/B/NK (5-color) in Rat Spleen

Panel Design

Purpose	Sample	Antibody Collocation
Adjust the voltage	1	Blank
Adjust compensation	2	CD3-Elab Fluor^® Red 780
	3	CD4-Elab Fluor^® Violet 450
	4	CD8-FITC
	5	CD45RA-PE
	6	CD161-APC
APC-FMO in combination with Isotype Control for auxiliary gating	7	CD3-Elab Fluor^® Red 780, CD4-Elab Fluor^® Violet 450, CD8-FITC, CD45RA-PE. Mouse IgG1, κ Isotype Control-APC
PE-FMO in combination with Isotype Control for auxiliary gating	8	CD3-Elab Fluor^® Red 780, CD4- Elab Fluor^® Violet 450, CD8-FITC, CD161-APC; Mouse IgG1, κ Isotype Control-PE
Full panel	9	CD3-Elab Fluor^® Red 780, CD4-Elab Fluor^® Violet 450, CD8-FITC, CD45RA-PE, CD161-APC

Information of Flow Cytometry Antibodies

Marker	Fluorochrome	Clone No.	Cat. No.
CD3	Elab Fluor^® Red 780	G4.18	E-AB-F1228S
CD4	Elab Fluor^® Violet 450	OX-38	E-AB-F1105Q
CD8	FITC	OX-8	E-AB-F1098C
CD45RA	PE	OX-33	E-AB-F1306D
CD161	APC	3.2.3	E-AB-F1307E
Mouse IgG1, κ Isotype Control	PE	MOPC-21	E-AB-F09792D
Mouse IgG1, κ Isotype Control	APC	MOPC-21	E-AB-F09792E

Flow Cytometry Result

Tips:

1. The CD3/CD4/CD8 cells are easy to be distinguished, and even without an Isotype Control, they can effectively distinguish between positive and negative cell populations.

2. Isotype Control for CD45RA and CD161 is suggested for auxiliary gating.

3. The phenotype of rat B cells is CD3^-CD45RA⁺, and the phenotype of NK cells is CD3^-CD161⁺.

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha

Cell Types

Detection of T/B/NK (5-color) in Rat Spleen

Panel Design

Information of Flow Cytometry Antibodies

Flow Cytometry Result

About Us

United States

Leave Message

Apply for

My Cart

Cell Types

Detection of T/B/NK (5-color) in Rat Spleen

Panel Design

Information of Flow Cytometry Antibodies

Flow Cytometry Result

About Us

Subscribe Us

United States

Leave Message

Apply for

My Cart