(KO Validated) EZH2 Polyclonal Antibody
Price: $ 580
Price: $ 360
Price: $ 220
- Host: Rabbit
- Reactivity: Human;Mouse
- Applications: WB;IF
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:HeLa Verified Samples in IF: U2OS |
Dilution |
WB 1:500-1:2000, IF 1:50-1:200 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant fusion protein of human EZH2 |
Abbre | EZH2 |
Synonyms | ENX-1;ENX1;EZH1;EZH2b;KMT6;KMT6A;WVS;WVS2;EZH2;KMT6 / EZH2 |
Swissprot | |
Calculated MW | 79kDa/81kDa/84kDa/85kDa/86kDa |
Observed MW |
78-110kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus |
Concentration | 1 mg/mL |
Buffer | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer;Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. |