Focudex G-25 Medium

    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
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    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience
    • G-25-moderate-Elabscience

      Catalog number:E-CM-GF09

      Size:
      • 25g
      • 100g
      • 500g
      • 1000g
      Qty:
      - +
      Price: $157

      Matrix: Dextran

      Particle size range: 45-165 µm

      Average particle size: 100-300 µm

      Pressure: ≤0.3 MPa

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Product introduction

      G-25 (medium) can be used to purify biological molecules of different molecular weight, which is based on the different crosslinking degree of media, and particle diameter and pore size after swelling. It is mainly applied in sample desalting, buffer exchange and removal of small molecular impurities.

       

      Advantages

      1. Easy operation: it can be connected with an injector or a pump, but also can be operated as a gravity column.

      2. Quick: the operation can be finished within 5~25 min.

      3. Significant effect: desalination rate >98%.

      4. High recovery rate: the recovery rate of protein is more than 85%-95%.

      5. Easy to be implemented: multiple series and other large size chromatography columns can be loaded according to requirements.

       

      Performance index

      Matrix

      Dextran

      Particle size range (dry powder)

      45-165 µm

      Particle size range (wet gel)

      100-300 µm

      Swelling degree

      4-6 mL/g

      Exclusion limit (Mr)

      5×103 Globular proteins

      pH stability

      2-13

      Void volume

      30%CV

      Loading amount (volume)

      5%-30%CV

      Chemical stability

      All common buffers

      Flow rate

       1 mL/min~15 mL/min (5 mL media)

      Pressure

      0.3 MPa

      Storage buffer

      20% Ethanol (for swelling media)

      Storage temperature

      4~30


      Application example

      Example 1:



      Note: The Example 1 shows that the retention time of the target sample varies with the volume of the sample. 

      Relevant data on the application effects of Example 1.

      Sample volume (mL)

      Collection volume (mL)

      Dilution ratio

      Desalination rate*

      Recovery rate**

      0.5

      1.2-1.5

      2.4-3.0

      >99

      >85

      1.0

      1.8-2.0

      1.8-2.0

      >98

      >90

      1.5

      2.0-2.4

      1.3-1.6

      >96

      >95

       Note:

      * The effect of desalination mainly depends on the time period of sample collection and the volume of the sample.

      **The recovery rate mainly depends on the property of sample, the time period of the sample collection and the measurement error (when the sample concentration is too low, the measurement error is greater).


      Example 2:



      Note: Example 1 and Example 2 show that the retention time and concentration of the target sample are not affected by the flow rate.


      Example 3:



      Note: Example 3 shows that the change of flow rate (within the flow rate range that the medium can tolerate) will not affect the concentration, recovery rate and dilution ratio of the target sample, and will slightly affect the desalination rate.

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