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NEDD8 Polyclonal Antibody

Cat:E-AB-60222
Manual MSDS

Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:various cell lines
Verified Samples in WB:Mouse brain,Mouse kidney,Rat kidney,Rat brain
Dilution

WB 1:500-1:2000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human NEDD8
Abbre NEDD8
Synonyms NEDD8;NEDD-8
Swissprot
Calculated MW 9kDa
Observed MW 12kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purification Method Affinity purification
Research Areas Cancer;Cell Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Nedd8 is an 81 amino acid Ub-like protein. It is conjugated to protein substrates in much the same way as Ub, using the E1, E2, E3 cascade. The specific E1 used is a heterodimer of APPBP1/UBA3. Two E2s have been discovered to follow called Ubc12 and Ube2F. Neddylation causes conformational change of the Cullin protein in the E3 called cullin-RING ligases (CRLs), which enhances the E3 Ub ligase activity of CRLs.