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NEFH Polyclonal Antibody

Cat:E-AB-62149
Manual MSDS
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Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IF
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Product Details
Verified Samples Verified Samples in WB:Mouse brain
Verified Samples in IF:C6,Mouse brain
Dilution

WB 1:500-1:2000, IF 1:50-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human NEFH
Abbre NEFH
Synonyms CMT2CC;NFH;NEFH
Swissprot
Calculated MW 105kDa/112kDa
Observed MW 150KDa/220KDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization axon,cytoplasm,cytoskeleton,neurofilament,postsynaptic density,postsynaptic intermediate filament cytoskeleton,Schaffer collateral - CA1 synapse
Concentration 1 mg/mL
Buffer PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Purification Method Affinity purification
Research Areas Cancer;Neuroscience;Signal transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.