Synonyms:NR3C3,Nuclear receptor subfamily 3 group C member 3,PGR,PR,PRA,PRB,PRGR,Progesterone receptor,Progestin receptor form A,Progestin receptor form B
|Synonyms||NR3C3,Nuclear receptor subfamily 3 group C member 3,PGR,PR,PRA,PRB,PRGR,Progesterone receptor,Progestin receptor form A,Progestin receptor form B|
|Application||IHC-p(Detection kit: E-IR-R217),IF|
|Recommended dilution||IHC 1:100-1:300, IF 1:100-200|
|Cellular localization||Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.|
|Tissue specificity||In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region-specific responses to hormonal stimuli.|
|Purification||Protein A purification|
|Storage instructions||Store at -20℃. Avoid freeze / thaw cycles.|
|Storage buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.4.|
|Background||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.|
Immunohistochemistry of paraffin-embedded Rat testis tissue using PGR Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse liver tissue using PGR Monoclonal Antibody at dilution of 1:200.