PML Polyclonal Antibody

Uniprot : P29590
    <
    >

      Catalog number:E-AB-60240

      Synonyms:Acure promyelocytic leukemia,inducer of,MYL,Pml,PML,PP8675,Probable transcription factor PML,Promyelocytic leukemia,Promyelocytic leukemia inducer of,Promyelocytic leukemia protein,Protein PML,RING finger protein 71,RNF 71,RNF71,TRIM 19,Tripartite motif protein TRIM19,Tripartite motif-containing protein 19

      Size:
      • 60μL
      • 120μL
      • 200μL
      Qty:
      - +
      Price: $129

      Host: Rabbit

      Reactivity: H,M,R

      Applications: WB,IHC,IF

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Overview

      Synonyms Acure promyelocytic leukemia,inducer of,MYL,Pml,PML,PP8675,Probable transcription factor PML,Promyelocytic leukemia,Promyelocytic leukemia inducer of,Promyelocytic leukemia protein,Protein PML,RING finger protein 71,RNF 71,RNF71,TRIM 19,Tripartite motif protein TRIM19,Tripartite motif-containing protein 19
      Swissprot P29590
      Source Rabbit
      Reactivity Human,Mouse,Rat
      Immunogen Recombinant protein of human PML
      Application WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R217),IF
      Recommended dilution WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
      Concentration 1 mg/mL
      Clonality Polyclonal

      Properties

      Cellular localization Nucleus > nucleoplasm. Cytoplasm. Nucleus > PML body. Nucleus > nucleolus. Endoplasmic reticulum membrane. Early endosome membrane. Sumoylated forms localize to the PML nuclear bodies. The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclear localization signal are cytoplasmic. Detected in the nucleolus after DNA damage. Sequestered in the cytoplasm by interaction with rabies virus phosphoprotein.
      Isotype IgG
      Purification Affinity purification
      Conjugation Unconjugated
      Storage instructions Store at -20℃. Avoid freeze / thaw cycles.
      Storage buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
      Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

      Images

      Western blot analysis of extracts of various cell lines with PML Polyclonal Antibody

      Immunohistochemistry of paraffin-embedded human gastric cancer with PML Polyclonal Antibody

      Immunohistochemistry of paraffin-embedded human uterine cancer with PML Polyclonal Antibody

      Immunofluorescence analysis of HeLa cells with PML Polyclonal Antibody

      Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML Polyclonal Antibody at dilution of 1:1000.

      submit review
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History


        People Also Bought

        Leave Message

        *
        *
        *
        *
        *
        *
        *
        Message submitted successfully !