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Recombinant 2019-nCoV Nucleocapsid Protein (His Tag)

Uniprot : QHD43423.2

Cat.No.:PKSR030485
Expression host: E.coli

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Description

Synonyms	2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Species	Virus
Expression_host	E.coli
Sequence	Met1-Ala419
Accession	QHD43423.2
Application	Immunogen(E-AB-V1011,E-AB-V1012)
Mol_Mass	49.4 kDa
AP_Mol_Mass	50-60 kDa
Tag	N-6His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per µg as determined by the LAL method.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Formulation	Supplied as a 0.2 μM filtered solution of 20mM HEPES, 100mM NaCl, pH 8.0.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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Reviews/Q&A

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Reviews
Q&A

K******************oSubmitted [ Apr 19 2021 ]

Asked: I would like to know the exact protein sequence of PKSR030485 (COVID19 N-protein). Can you send it to me? Kind regards, Kirsten van Leijenhorst

adminSubmitted [ Apr 19 2021 ]

Answered: Description: Recombinant 2019-nCoV Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus. Accession: QHD43423.2 Amino Acid Sequence: HHHHHHMSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

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