Click here to view cell apoptosis assay products

5× SDS Loading Buffer

    • 5 × SDS Loading Buffer-Elabscience
    • 5 × SDS Loading Buffer-Elabscience
    • 5 × SDS Loading Buffer-Elabscience

      Catalog number:E-BC-R288

      • 2 mL
      • 5 mL
      • 10 mL
      - +
      Price: $20

      Lead Time:Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual


      This product is a loading buffer for protein samples with SDS-PAGE electrophoresis. The SDS contained in the product can be combined with the protein to form a SDS-protein complex, which bring a large amount of negative charge to the protein; SDS can break intramolecular and intermolecular hydrogen bonds, and destroy the secondary and the tertiary structure of protein. The DTT contained in the preparation can break the disufide bond between the cysteine residues, destroy the structure between the proteins, and eliminate the difference between the protein structures. Ultimately, the rate of protein migration in the SDS-PAGE is only related to its molecular weight. Bromophenol blue is used as an indicator for electrophoresis to determine the progress of electrophoresis.





      2 mL

      5 mL

      10 mL



      5× SDS Loading Buffer

      1 mL × 2

      1 mL × 5

      1 mL × 10



      1.Dissolve 5 × SDS Loading Buffer at room temperature or with water bath.

      2.For each 20 µL protein samples, add 5 µL 5 × SDS Loading Buffer and mix them well.

      3.Heat at 95~100℃ for 10 min to fully denatured the proteins.

      4.Centrifuge at 12000 rpm for 2 min, and collect the supernatant.

      5.Take 10~20 µL supernatant and add it into the SDS-PAGE gel well.


      5 XSDS Loading Buffer Components

      0.25 M Tris•HCl (pH6.8), 0.5 M DTT, 10% SDS, 0.5% BPB, 50% Glycerin.


      Store at -20°C for 12 months.



      1.When the product is stored at -20°C, SDS precipitation may occur. Please dissolve it in warm water before use. Please store them properly according to its usage.

      2.If there is still a relatively viscous or viscous translucent object after sample boiling, please extend the boiling time or add 1 × SDS loading buffer and boil the sample again, so that the genomic DNA bound to the protein can be partially broken to reduce the generation of sample viscosity.

      3.This product contains DTT, which is slightly irritating and has certain toxicity.


      1. Eugenol protects against Aspergillus fumigatus keratitis by inhibiting inflammatory response and reducing fungal load
        IF: 4.432
        DOI: 10.1016/j.ejphar.2022.174955
        PMID: 35436473
        Products: E-BC-R288 
      2. Long-Term Wi-Fi Exposure From Pre-Pubertal to Adult Age on the Spermatogonia Proliferation and Protective Effects of Edible Bird’s Nest Supplementation
        IF: 4.566
        Journal: Frontiers in Physiology(2022)
        DOI: 10.3389/fphys.2022.828578
        Products: E-BC-R288 
      3. Impaired Function of a Rare Mutation in the MMUT Gene Causes Methylmalonic Acidemia in a Chinese Patient
        IF: 1.375
        Journal: Genetics Research(2022)
        DOI: 10.1155/2022/5611697
        PMID: 35919035
        Products: E-BC-R288 
      Show AllShorten
      • Show all (1)
      • Reviews (0)
      • Q&A (1)

      M************* Submitted [ Sep 17 2022 ]

      Asked: Hello dear .. i hope this finds you well . Regarding this loading buffer , i use it recently and i faced some concerns . in directions number 2 , by protein sample did you mean sample alone or diluted with distilled water ? i used it according to BCA protein calculation ( 30ug/well) so i add ( 5.87ul of pt sample + 14.13ul dH2O ) to be 20ul + 5ul of loading buffer . Actually i get high bands intensity , i am doubt in this issue .. Kindly please guid me to overcome this . Best Regards .



      adminSubmitted [ Sep 17 2022 ]

      Answered: Hi Dear, Thanks for your feedback. Whether the protein sample needs to be diluted or not needs to be determined according to the concentration detected by BCA. If the extracted protein concentration is greater than 5mg/mL, it can be diluted appropriately with PBS to make the protein concentration around 4mg/mL before sample preparation. If the extracted protein concentration is lower than 5mg/mL, loading buffer can be directly applied to prepare samples according to the proportion specified in the instructions.

      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History

        People Also Bought

        Apply for
        *Product Name:
        *Catalog Number:
        *When will you use it?