English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

SYNCRIP Polyclonal Antibody

Cat:E-AB-61591
Manual MSDS
  • +2

Price: $ 530

Price: $ 320

Price: $ 200

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:various cell lines
Verified Samples in IHC:Human liver cancer,Mouse intestine,Mouse kidney
Dilution

WB 1:500-1:2000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human SYNCRIP
Abbre SYNCRIP
Synonyms SYNCRIP;GRY-RBP;GRYRBP;HNRNPQ;HNRPQ1;NSAP1;PP68;hnRNP-Q
Swissprot
Calculated MW 46kDa/58kDa/62kDa/65kDa/69kDa
Observed MW 50-100kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm,Endoplasmic reticulum,Microsome,Nucleus,Nucleus,nucleoplasm
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Purification Method Affinity purification
Research Areas Cancer;Epigenetics and Nuclear Signaling;Microbiology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 20.