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Yeast Expression

Yeast protein expression system is the most economical eukaryotic expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins.


Elabscience's Yeast Protein Expression System Service start with gene analysis (S-CP-Y001), followed by subcloning (S-CP-Y002), screening of expression strains and small amount protein expression (S-CP-Y003). According to your project, we can offer the target protein in large scale (1~5L, S-CP-Y004 or 1~30L, S-CP-Y005). Results of each service will be delivered with our identification reports to keep you informed of the progress. You can order every single service or any combination of these services according to your project program.

Submit your project information and requirements and place your order, our experienced experts will help with your research and promise you the high quality services with competitive price.


Advantages

Proprietary Technologies: Our advanced protein platform considers promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location, which are essential for high-level recombinant protein production.

Humanized antibody production: Elabscience® has developed a platform that produces humanized antibodies in yeast expression system. The similarity between the yeast and mammalian system for recombinant antibody expression enables Elabscience® to reduce the high cost associated with manufacturing recombinant antibody in mammalian expression system.

Streamlined Services: Elabscience® can take your project directly from gene synthesis, to the downstream contents including recombinant protein expression, antibody production, and assay development.


Yeast protein expression and purification services (Pichia pastoris System) Details

Cat. No.

Service Name

Specification

Timeline

Delivery

Quoted price

S-CP-Y001

Analysis of gene and target protein

  • Codon optimization, protein hydrophilic, hydrophobic, PI, structural domain, etc

 1-2 days

  • Analysis of results

Free

S-CP-Y002

Subcloning

  • Gene synthesis
  • Construction of expression vector
  • Plasmids Prep and analysis

3-4 weeks

  • Expression vector containing the target gene
  • Target sequencing report.
  • E.coli containing plasmids of the expression vector
  • Plasmid instructions

$0.35/bp

 

S-CP-Y003

Screening of expression strains

  • Amplification of recombinant plasmids  and linear  electrotransformation of Pichia pastor with the recombinant plasmids
  • Obtain high-copy strain by antibiotic screening
  • Small amount expression with ten selected positive transformation strains and protein expression evaluation and optimization
  • SDS-PAGE to identify the expression

3-4 weeks

  • SDS-PAGE analysis results
  • Expression strain (extra charge, $1500 / strain)

$650

S-CP-Y004

Protein expression and purification in large scale

  • Large amount expression with selected strains of high-expression
  • Protein purification
  • SDS-PAGE test

2-3 weeks

  • Purified recombinant protein (purity >90%)
  • Related analysis reports

$580

S-CP-Y005

High density cultivation of protein expression in fermentation tanks and purification

  • Carry out the experiment with the optimized expression condition
  • Protein purification
  • SDS-PAGE test

3-4 weeks

  • Ultra large amount of purified protein (We have fermentation tanks of 10L and 30L, thus high-purified protein up to gram level can be delivered)
  • Related analysis reports

Inquire


Quotations and Ordering

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