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Current Position:Home>>Kits>>ELISA Kits>> Rat AMH(Anti-Mullerian Hormone) ELISA Kit

Rat AMH(Anti-Mullerian Hormone) ELISA Kit

Citations(23)

  • Cat.No.:E-EL-R3022

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat AMH in samples. No significant cross-reactivity or interference between Rat AMH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat AMH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AMH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AMH. You can calculate the concentration of Rat AMH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat AMH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat AMH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 164.78 488.04 1854.15 191.52 482.82 1632.48
Standard deviation 9.72 23.91 79.73 11.17 21.82 78.20
CV (%) 5.90 4.90 4.30 5.83 4.52 4.79

Recovery

The recovery of Rat AMH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-99 95
EDTA plasma (n=8) 90-99 95
Cell culture media (n=8) 92-107 99

Linearity

Samples were spiked with high concentrations of Rat AMH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsMIF, MIH, MIS
Research AreaCancer, Developmental biology, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. CARBOHYDRATE POLYMERS (2022) IF: 9.381
    Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota

    DOI: 10.1016/j.carbpol.2022.119304

    PMID: 35422283

    		 Sample: serum
  2. Stem Cell Research & Therapy (2022) IF: 8.0790
    miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failure

    DOI: 10.1186/s13287-022-03056-y

    PMID: 35883161

    		 Sample: serum
  3. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129
    Arsenic exposure diminishes ovarian follicular reserve and induces abnormal steroidogenesis by DNA methylation

    DOI: 10.1016/j.ecoenv.2022.113816

    PMID: 36068745

    		 Sample: Serum
  4. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Can a Large Number of Transplanted Mesenchymal Stem Cells Have an Optimal Therapeutic Effect on Improving Ovarian Function?

    DOI: 10.3390/ijms232416009
    Sample: serum
  5. LIFE SCIENCES (2022) IF: 6.78
    Moxibustion mitigates mitochondrial dysfunction and NLRP3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats

    DOI: 10.1016/j.lfs.2022.121283
    Sample: serum
  6. Stem Cell Research & Therapy (2022) IF: 6.832
    Increased phosphatase regenerating liver-1 trigger vascular remodeling in injured ovary via platelet-derived growth factor signaling pathway

    DOI: 10.1186/s13287-022-02772-9

    PMID: 35255961

    		 Sample: serum
  7. Antioxidants (2020) IF: 5.014
    Placenta-Derived Mesenchymal Stem Cells Restore the Ovary Function in an Ovariectomized Rat Model via an Antioxidant Effect

    DOI: 10.3390/antiox9070591

    PMID: 32640638

    		 Sample: Serum
  8. Stem Cell Research & Therapy (2020) IF: 5.116
    Microenvironmental changes induced by placenta-derived mesenchymal stem cells restore ovarian function in ovariectomized rats via activation of the PI3K-FOXO3 pathway

    DOI: 10.1186/s13287-020-02002-0

    PMID: 33198818

    		 Sample: Serum,Tissue homogenate
  9. Aging-US (2021) IF: 5.682
    Paeoniflorin attenuates DHEA-induced polycystic ovary syndrome via inactivation of TGF-β1/Smads signaling pathway in vivo

    DOI: 10.18632/aging.202564

    PMID: 33638949

    		 Sample: Serum
  10. Drug Design Development and Therapy (2018) IF: 4.162
    Examination of the effect of ovarian radiation injury induced by hysterosalpingography on ovarian proliferating cell nuclear antigen and the radioprotective effect of amifostine: an experimental study

    DOI: 10.2147/DDDT.S156757
    Sample: Serum
  11. Journal of Ovarian Research (2021) IF: 4.234
    Ameliorative effect of recombinant human lactoferrin on the premature ovarian failure in rats after cyclophosphamide treatments

    DOI: 10.1186/s13048-020-00763-z

    PMID: 33478578

    		 Sample: Serum
  12. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Intermittent hypobaric hypoxia causes deleterious effects on the reproductive system in female rats

    DOI: 10.1016/j.biopha.2020.110511

    PMID: 32679462

    		 Sample: Serum
  13. Cannabis and Cannabinoid Research (2023) IF: 4.786
    2-Arachidonoylglycerol Activity in Over Ischemia Reperfusion Damage: Can Endocannabinoids Protect Ovarian Reserve?

    DOI: 10.1089/can.2022.0242
    Sample: Serum,ovary tissues
  14. REPRODUCTION (2020) IF: 3.206
    Irisin may have a role in pubertal development and regulation of reproductive function in rats

    DOI: 10.1530/REP-20-0072
    Sample: serum
  15. Life-Basel (2022) IF: 3.251
    Rescue IVM of Denuded GV- and MI-Stage Oocytes of Premenopausal Rats with Oncostatin M, Insulin-like Growth Factor I, and Growth Hormone

    DOI: 10.3390/life12081247

    PMID: 36013426

    		 Sample: serum
  16. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Ameliorative effect of nicorandil in ovarian ischemia-reperfusion–induced injury in rats: role of potassium channel

    DOI: 10.1007/s00210-020-01854-w
    Sample: Serum
  17. TOXICOLOGY MECHANISMS AND METHODS (2020) IF: 2.295
    Cilostazol protects against cyclophosphamide-induced ovarian toxicity in female rats: role of cAMP and HO-1

    DOI: 10.1080/15376516.2020.1774829

    PMID: 32456565

    		 Sample: Serum
  18. REPRODUCTION FERTILITY AND DEVELOPMENT (2022) IF: 2.311
    Capsaicin prevents radiotherapy-induced premature ovarian failure in rats

    DOI: 10.1071/RD21235

    PMID: 35101163

    		 Sample: serum
  19. TOXICOLOGY AND INDUSTRIAL HEALTH (2018) IF: 1.255
    The impact of electromagnetic radiation (2.45 GHz, Wi-Fi) on the female reproductive system: The role of vitamin C:

    DOI: 10.1177/0748233718775540
    Sample: Serum
  20. TOXICOLOGY AND INDUSTRIAL HEALTH (2018) IF: 1.255
    Pathology of cigarettes on the reproductive system and ameliorative effects of alpha lipoic acid: A rat model study:

    DOI: 10.1177/0748233718755160
    Sample: Serum
  21. Journal of Clinical Research in Pediatric Endocrinology (2020) IF: 1.803
    Effects of 5-Hydroxymethylfurfural on Pubertal Development of Female Wistar Rats

    DOI: 10.4274/jcrpe.galenos.2019.2019.0080

    PMID: 31475510

    		 Sample: Serum
  22. ACUPUNCTURE & ELECTRO-THERAPEUTICS RESEARCH (2021) IF: 0.143
    The Effectiveness of Electro-acupuncture in Ovarian Ischemia Reperfusion Injury

    DOI: 10.3727/036012921X16264625521781
    Sample: serum
  23. ULTRASTRUCTURAL PATHOLOGY (2020) IF: 0.989
    Investigation of the preventive effects of dehydroepiandrosterone (DHEA) and Caffeic acid phenethyl ester (CAPE) on cisplatin-induced ovarian damage in rats

    DOI: 10.1080/01913123.2019.1711479
    Sample: serum
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