Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD119 Antibody[GR-20]
Cat.No.:E-AB-F11150
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Alternate Names | Interferon gamma receptor 1,Ifngr1,IFN-gamma-R1,IFN-gamma-R-alpha,CD119 |
Swissprot | P15261 |
References |
1. Basu M, et al. 1988. P. Natl. Acad. Sci. USA 85:6282.
2. Codias EK, et al. 1990. J. Immunol. 144:2197. |
Form | Liquid |
Clone No. | GR-20(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CDw119 is a 90 kD immunoglobulin superfamily member, also known as IFN-γRα chain. It is a class II cytokine receptor family member that serves as a IFN-γ-binding chain associated with the IFN-γ β chain also known as AF-1. In addition to ligand binding, CDw119 participates in ligand trafficking. CDw119 is expressed on T and B cells, NK cells, fibroblasts, endothelial, and epithelial cells. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Mouse CD119 Antibody[GR-20] | 25μg, 100μg | Check More |
Biotin Anti-Mouse CD119 Antibody[GR-20] | 25μg, 100μg | Check More |
PE Anti-Mouse CD119 Antibody[GR-20] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD119 Antibody[GR-20] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD119 Antibody[GR-20] | 25μg, 100μg | Check More |
APC Anti-Mouse CD119 Antibody[GR-20] | 25μg, 100μg | Check More |