Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse TCRβ Antibody[H57-597 (HB218)]
Cat.No.:E-AB-F11230
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Alternate Names | TCR-β chain, TCR-β, β-TCR |
References |
1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749. 3. Kubo R, et al. 1989. J. Immunol. 142:2736. |
Form | Liquid |
Clone No. | H57-597 (HB218)(See Other Available Formats) |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Isotype Control | AF/LE Purified Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F098530] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |