Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> APC Anti-Human CD28 Antibody[CD28.2]
Cat.No.:E-AB-F1195E
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | PAS2,MNS,HGpMiX,GYPA,GPSAT |
Swissprot | P10747 |
References |
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191. |
Form | Liquid |
Clone No. | CD28.2(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | APC |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Human CD28 Antibody[CD28.2] | 50μg, 500μg, 1mg | Check More |
Purified Anti-Human CD28 Antibody[CD28.2] | 25μg, 100μg | Check More |
FITC Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Elab Fluor® 594 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |