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Cat.No.:E-AB-F1205UE
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Alternate Names | Interleukin-5,IL-5,B-cell differentiation factor I,T-cell replacing factor,TRF |
Swissprot | P05113,P04401 |
References |
1. Fitzgerald, K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K, et al. 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immunol. 35:593. |
Form | Liquid |
Clone No. | TRFK5(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | APC Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823E] |
Reactivity | Human,Mouse |
Application | ICFCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | APC |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5. |
Related Protocols | Staining Intracellular Antigens for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Human/Mouse IL-5 Antibody[TRFK5] | 50μg, 500μg, 1mg | Check More |
Purified Anti-Human/Mouse IL-5 Antibody[TRFK5] | 25μg, 100μg | Check More |
PE Anti-Human/Mouse IL-5 Antibody[TRFK5] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human/Mouse IL-5 Antibody[TRFK5] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human/Mouse IL-5 Antibody[TRFK5] | 25μg, 100μg | Check More |