Alternate Names	Lymphocyte antigen 6G,Ly-6G,Ly-6G.1,Ly6g
Swissprot	P35461
References	1.Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. J Immunol. 1993; 151(5):2399-2408. 2.Hestdal K, Ruscetti FW, Ihle JN, et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991;147(1):22-28. 3.Jutila MA, Kroese FG, Jutila KL, et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. Eur J Immunol. 1988; 18(11):1819-1826. 4.Lagasse E, Weissman IL. Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods. 1996; 197(1-2):139-150.

Product Details

Form	Liquid
Clone No.	1A8(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	APC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832E]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	APC
	APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse Ly6G Antibody[1A8]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
Biotin Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
FITC Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly6G Antibody[1A8]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PE Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
APC Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PerCP Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly6G Antibody[1A8]	25μg, 100μg	Check More

Citations

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 6.53
Epigallocatechin-3-gallate protects immunity and liver drug-metabolism function in mice loaded with restraint stress

DOI: 10.1016/j.biopha.2020.110418

Sample: Whole Blood
Scientific Reports (2022) IF: 4.996
Changes of macrophage and CD4+ T cell in inflammatory response in type 1 diabetic mice

DOI: 10.1038/s41598-022-19031-9

PMID: 36056051
Sample: Spleen tissue

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