BV2 Cell Line


      Catalog number:EP-CL-0493

      • 1×10⁶ cells/vial
      - +
      Price: $461

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Origin and General Characteristics

      Cell Name BV2 Cell Line
      Organism Mus musculus, Mouse
      Tissue Brain
      Morphology Morphology microglial
      Growth Properties Mixed, Adherent And Suspension
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium MEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

      Related Resources

      Recommendations for handling of cryopreserved cells

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      • Q&A (1)

      S***********gSubmitted [ Dec 09 2020 ]

      Asked: May I please get a quotation for this cell line? Thank you very much. Address: Department of Biology Hong Kong Baptist University Rm OEW1002, 10/F, Oen Hall Building 224 Waterloo Road, Kowloon Tong



      adminSubmitted [ Dec 09 2020 ]

      Answered: EP-CL-0493 BV2 Cell Line 1×10⁶ cells/vial $461 We will send a spare vial for research support. shipping cost$80 with dry ice via FedEx priority alert service; The lead time is in 3 business days after receiving payment. If any further question, pls feel free to contact me. Britney +86 17764206645 (wechat)

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