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Catalog number:EP-CL-0493
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Add to cart Compare Bulk requestCell Name | BV2 Cell Line |
Organism | Mus musculus, Mouse |
Tissue | Brain |
Morphology | Morphology microglial |
Growth Properties | Mixed, Adherent And Suspension |
Biosafety Level | 1 |
Complete Growth Medium | MEM +10% FBS +1% P/S |
Subculturing | Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
Subcultivation Ratio | 1:2-1:4 |
Medium Renewal | Every 2 to 3 days |
Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃ |
Recommendations for handling of cryopreserved cells
S***********gSubmitted [ Dec 09 2020 ]
Asked: May I please get a quotation for this cell line? Thank you very much. Address: Department of Biology Hong Kong Baptist University Rm OEW1002, 10/F, Oen Hall Building 224 Waterloo Road, Kowloon Tong
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adminSubmitted [ Dec 09 2020 ]
Answered: EP-CL-0493 BV2 Cell Line 1×10⁶ cells/vial $461 We will send a spare vial for research support. shipping cost$80 with dry ice via FedEx priority alert service; The lead time is in 3 business days after receiving payment. If any further question, pls feel free to contact me. Britney britney@elabscience.com +86 17764206645 (wechat)