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BV2 Cell Line

Cat.No.:EP-CL-0493

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1×10⁶ cells/vial

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Origin and General Characteristics

Cell Name	BV2 Cell Line
Organism	Mus musculus, Mouse
Tissue	Brain
Morphology	Morphology microglial
Growth Properties	Mixed, Adherent And Suspension
Biosafety Level	1

Culture Conditions and Handling

Complete Growth Medium	MEM(EP-CM-L0139)+10% FBS+1% P/S(EP-CM-L0048) (BV2 Cell Line complete medium (EP-ML-0493) is recommended.)
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Related Products

EP-CM-P0139	MEM (with NEAA), powder
EP-CM-L0139	MEM (with NEAA)
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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Reviews/Q&A

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Reviews
Q&A

Z*eSubmitted [ Jul 27 2022 ]

Asked: May I know how many passages of BV2 are recommended to use?

adminSubmitted [ Jul 27 2022 ]

Answered: In general, the number of passage is not a decisive factor when choosing a cell line as an experimental subject. This cell line was established in 1990 and has been passaged many times so far. It is recommended that you subculture 1-2 times after receiving the cells, and adjust the state of the cells to adapt to the new culture environment before conducting subsequent experiments.

P****************gSubmitted [ May 30 2022 ]

Asked: Dear Elabscience My name is Panlekha Rungruang, the fourth years student in Molecular Medicine program, which is under Faculty of Science, Faculty of Medicine Ramathibody hospital, Faculty of Medicine Siriraj hospital, Faculty of Dentistry and Faculty of Tropical medicine, Mahidol university, Thailand. I’m writing to follow up on the information about BV-2 cell line. If I would like to purchase the BV2 cell line, how to purchase or which agency in Thailand could I contact and the price of this cell line. With kind regards, Panlekha Rungruang

adminSubmitted [ May 30 2022 ]

Answered: Hi Panlekha Rungruang, This is Lizzy from Elabscience. Nice to meet you^_^ Pls contact our distributor Mr Chotipat from Pacific Science. Pacific Science Co.,Ltd. Chotipat Adress: 62, 64 Soi Charansanitwong 49/1, Bangnumru Bangplad, Bangkok, 10700 THAILAND Tel: 6624330068-9 Fax: 6624342609 Email : pacscien@ksc.th.com Website : www.pacificscience.co.th

M**************dSubmitted [ Mar 18 2021 ]

Asked: Could I please get a quotation for this cell line? BV2 microglia cells EP-CL-0493.

adminSubmitted [ Mar 18 2021 ]

Answered:

adminSubmitted [ Mar 18 2021 ]

Answered: Hello Markus, our distributor in the US will contact you to send the quote. Thank you.

C**********tSubmitted [ Jan 29 2021 ]

Asked: Can you provide any references where these cells were used?

adminSubmitted [ Jan 29 2021 ]

Answered: Dear Corena, Thanks for your support. Sorry to tell you we didn't collect the references for this cell line. If you have any questions, pls feel free to ask. Best regards,

S***********gSubmitted [ Dec 09 2020 ]

Asked: May I please get a quotation for this cell line? Thank you very much. Address: Department of Biology Hong Kong Baptist University Rm OEW1002, 10/F, Oen Hall Building 224 Waterloo Road, Kowloon Tong

adminSubmitted [ Dec 09 2020 ]

Answered: EP-CL-0493 BV2 Cell Line 1×10⁶ cells/vial $461 We will send a spare vial for research support. shipping cost$80 with dry ice via FedEx priority alert service; The lead time is in 3 business days after receiving payment. If any further question, pls feel free to contact me. Britney britney@elabscience.com +86 17764206645 (wechat)

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