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BV2 Cell Line

Cat.No.:EP-CL-0493

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1×10⁶ cells/vial

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Origin and General Characteristics

Cell Name	BV2 Cell Line
Organism	Mus musculus, Mouse
Tissue	Brain
Morphology	Morphology microglial
Growth Properties	Mixed, Adherent And Suspension
Biosafety Level	1

Culture Conditions and Handling

Complete Growth Medium	MEM +10% FBS +1% P/S (BV2 Cell Line complete medium (EP-ML-0493) is recommended.)
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

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EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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Reviews/Q&A

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Reviews
Q&A

M**************dSubmitted [ Mar 18 2021 ]

Asked: Could I please get a quotation for this cell line? BV2 microglia cells EP-CL-0493.

adminSubmitted [ Mar 18 2021 ]

Answered:

adminSubmitted [ Mar 18 2021 ]

Answered: Hello Markus, our distributor in the US will contact you to send the quote. Thank you.

C**********tSubmitted [ Jan 29 2021 ]

Asked: Can you provide any references where these cells were used?

adminSubmitted [ Jan 29 2021 ]

Answered: Dear Corena, Thanks for your support. Sorry to tell you we didn't collect the references for this cell line. If you have any questions, pls feel free to ask. Best regards,

S***********gSubmitted [ Dec 09 2020 ]

Asked: May I please get a quotation for this cell line? Thank you very much. Address: Department of Biology Hong Kong Baptist University Rm OEW1002, 10/F, Oen Hall Building 224 Waterloo Road, Kowloon Tong

adminSubmitted [ Dec 09 2020 ]

Answered: EP-CL-0493 BV2 Cell Line 1×10⁶ cells/vial $461 We will send a spare vial for research support. shipping cost$80 with dry ice via FedEx priority alert service; The lead time is in 3 business days after receiving payment. If any further question, pls feel free to contact me. Britney britney@elabscience.com +86 17764206645 (wechat)

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