BV2 Cell Line

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      Catalog number:EP-CL-0493

      Size:
      • 1×10⁶ cells/vial
      Qty:
      - +
      Price: $401

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Origin and General Characteristics

      Cell Name BV2 Cell Line
      Organism Mus musculus, Mouse
      Tissue Brain
      Morphology Morphology microglial
      Growth Properties Mixed, Adherent And Suspension
      Biosafety Level 1

      Culture Conditions and Handling

      Complete Growth Medium MEM +10% FBS +1% P/S
      Subculturing Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
      Subcultivation Ratio 1:2-1:4
      Medium Renewal Every 2 to 3 days
      Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
      Culture Conditions Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

      Related Resources

      Recommendations for handling of cryopreserved cells

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