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BV2 Cell Line

Catalog number:EP-CL-0493

Size:

1×10⁶ cells/vial

Price: $461

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Datasheet
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Origin and General Characteristics

Cell Name	BV2 Cell Line
Organism	Mus musculus, Mouse
Tissue	Brain
Morphology	Morphology microglial
Growth Properties	Mixed, Adherent And Suspension
Biosafety Level	1

Culture Conditions and Handling

Complete Growth Medium	MEM +10% FBS +1% P/S
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Related Resources

Recommendations for handling of cryopreserved cells

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Q&A (1)

S***********gSubmitted [ Dec 09 2020 ]

Asked: May I please get a quotation for this cell line? Thank you very much. Address: Department of Biology Hong Kong Baptist University Rm OEW1002, 10/F, Oen Hall Building 224 Waterloo Road, Kowloon Tong

adminSubmitted [ Dec 09 2020 ]

Answered: EP-CL-0493 BV2 Cell Line 1×10⁶ cells/vial $461 We will send a spare vial for research support. shipping cost$80 with dry ice via FedEx priority alert service; The lead time is in 3 business days after receiving payment. If any further question, pls feel free to contact me. Britney britney@elabscience.com +86 17764206645 (wechat)

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