BV2 Cell Line

Name: BV2 Cell Line
Brand: Elabscience
SKU: EP-CL-0493
Price: 401 USD
Availability: InStock

Catalog number:EP-CL-0493

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Price: $401

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Cell Name	BV2 Cell Line
Organism	Mus musculus, Mouse
Tissue	Brain
Morphology	Morphology microglial
Growth Properties	Mixed, Adherent And Suspension
Biosafety Level	1

Complete Growth Medium	MEM +10% FBS +1% P/S
Subculturing	Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of 0.25% (w/v) Trypsin-0.53mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	1:2-1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

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