The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

General Information

Product Form	Liquid
Concentration	1×
pH	7.2-7.4
D-Glucose	3151 mg/L
HEPES	15mM
L-glutamine	2.5mM
NaHCO₃	1200 mg/L
Phenol red	8.1 mg/L
Sodium pyruvate	0.5mM
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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