ENR (Enrofloxacin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E032

      Size:
      • 96T
      Qty:
      - +
      Price: $260

      sample_type: Tissue,Honey,Milk,Milk powder,Eggss.

      Sensitivity: 0.1 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Enrofloxacin (ENR) in samples, such ashoney, tissue, milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, ENR in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-ENR antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of ENR. The concentration of ENR in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.1 ppb (ng/mL)

      Reaction mode: 25,45 min~15min

      Detection limit: Tissue---0.3 ppb; Honey ---0.4 ppb; Milk---3 ppb; Milk powder---6 ppb;

         Eggs---3 ppb.

      Cross-reactivity: Enrofloxacin---100%, Oxolinic acid---28%, Levofloxacin--10%, Lomefloxacin---4%, Marbofloxacin---4%, Sarafloxacin---2%

      Sample recovery rate:  85%±15%.



      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1mL each

      (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)

      HRP Conjugate

      5.5 mL

      Antibody Working Solution 

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution 

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      5×Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy

      Other supplies required

      Instrument: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01g), 

      High-precision transferpettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL) 

      Reagents: Anhydrous acetonitrile, N-hexane, Concentrated HCl, Dichloromethane



      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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