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Cat.No.:E-AB-F1197C
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Alternate Names | Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF |
Swissprot | P18893 |
References |
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefy R, et al. 1992. Curr. Opin. Immunol. 4:314. 3. Howard M, et al. 1992. Immunol. Today 13:198. 4. Quesniaux V. 1992. Res. Immunol. 143:385. 5. Norton SK, et al. 2008. J. Immunol. 180:2848. |
Form | Liquid |
Clone No. | JES5-16E3(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | FITC Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842C] |
Reactivity | Mouse |
Application | ICFCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | FITC |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
L-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-γ, TNF-β, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-α synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4. |
Related Protocols | Staining Intracellular Antigens for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
PE Anti-Mouse IL-10 Antibody[JES5-16E3] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse IL-10 Antibody[JES5-16E3] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Mouse IL-10 Antibody[JES5-16E3] | 25μg, 100μg | Check More |
PE Anti-Mouse IL-10 Antibody[JES5-16E3] | 25μg, 100μg | Check More |
APC Anti-Mouse IL-10 Antibody[JES5-16E3] | 25μg, 100μg | Check More |