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Human AP13(Apelin 13) ELISA Kit

  • Cat.No.:E-EL-H0458

  • Reactivity: Human

To Purchase E-EL-H0458

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 125.00-8000 pg/mL
Sensitivity 75.00 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes AP13 in samples. No significant cross-reactivity or interference between AP13 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of AP13 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with AP13. During the reaction, AP13 in the sample or standard competes with a fixed amount of AP13 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to AP13. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AP13 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level AP13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level AP13 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 426.70 1074.70 3419.80 465.10 1177.90 3157.70
Standard deviation 28.20 51.60 143.60 31.60 56.50 132.60
CV (%) 6.61 4.80 4.20 6.79 4.80 4.20


The recovery of AP13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 98
EDTA plasma(n=8) 93-110 101
Cell culture media(n=8) 95-108 100


Samples were spiked with high concentrations of Human AP13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q9ULZ1
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Translational Stroke Research (2020) IF: 5.78
    The Association Between Serum Apelin-13 and the Prognosis of Acute Ischemic Stroke

    DOI: 10.1007/s12975-019-00769-w

    Sample: serum
  2. CLINICA CHIMICA ACTA (2021) IF: 3.786
    Serum apelin-13 and risk of death following severe traumatic brain injury

    DOI: 10.1016/j.cca.2021.01.014

    PMID: 33508251

    Sample: Serum
    Exploratory study to evaluate plasma vasopressin and apelin‐13 levels in children with attention‐deficit hyperactivity disorder

    DOI: 10.1111/pcn.12415

    Sample: Plasma
    Evaluation of Apelin-13 levels in patients with diabetic nephropathy

    DOI: 10.1007/s11255-022-03323-0

    PMID: 35925488

    Sample: serum
    Evaluation of serum apelin‐13 and apelin‐36 concentrations in preeclamptic pregnancies

    DOI: 10.1111/jog.14137

    Sample: Serum
  6. OPHTHALMOLOGICA (2020) IF: 1.926
    Apelin-13: A Promising Biomarker for Age-Related Macular Degeneration?

    DOI: 10.1159/000513050

    Sample: serum
  7. Arterial Hypertension (2022)
    Peculiarities Of Changes Of Apelin-13 Concentration In Patients With Essential Hypertension And Extrasystole

    DOI: 10.5603/AH.a2022.0018

    Sample: serum
  8. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2020) IF: 0.373
    The investigation effect of weight loss on serum vaspin, apelin-13, and obestatin levels in obese individual

    DOI: 10.1515/tjb-2019-0143

    Sample: serum
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