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Human CEA(Carcinoembryonic Antigen) ELISA Kit

Citations(7)Uniprot : P06731

Cat.No.:E-EL-H6047
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human CEA in samples. No significant cross-reactivity or interference between Human CEA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human CEA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CEA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CEA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CEA. You can calculate the concentration of Human CEA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CEA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CEA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.49	1.20	4.32	0.50	1.04	4.59
Standard deviation	0.03	0.06	0.18	0.03	0.05	0.19
CV (%)	6.12	5.00	4.17	6.00	4.81	4.14

Recovery

The recovery of Human CEA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-104	96
EDTA plasma (n=8)	93-103	96
Cell culture media (n=8)	92-101	95

Linearity

Samples were spiked with high concentrations of Human CEA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P06731
Synonyms	CD66E, CD66, CEACAM5, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Research Area	Cancer, Immunology, Stem cells, Tags and Cell Markers

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOSENSORS & BIOELECTRONICS (2022) IF: 10.618
Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

DOI: 10.1016/j.bios.2022.114487

PMID: 35780540
Sample: exosomes
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020) IF: 9.4120
Development of a serum miRNA panel for detection of early stage non-small cell lung cancer

DOI: 10.1073/pnas.2006212117

PMID: 32943537
Sample: Serum
TALANTA (2021) IF: 6.057
Fluorescence-infrared absorption dual-mode nanoprobes based on carbon dots@SiO2 nanorods for ultrasensitive and reliable detection of carcinoembryonic antigen

DOI: 10.1016/j.talanta.2021.122342

Sample: Serum
European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
Hsa_circ_0124055 and hsa_circ_0101622 regulate proliferation and apoptosis in thyroid cancer and serve as prognostic and diagnostic indicators

DOI: 10.26355/eurrev_202004_21016

PMID: 32373972
Sample: Serum,Tissue homogenate
ANALYST (2020) IF: 3.978
In situ amplified QCM immunoassay for carcinoembryonic antigen with colorectal cancer using horseradish peroxidase nanospheres and enzymatic biocatalytic precipitation

DOI: 10.1039/D0AN01399D

Sample: serum
Analytical Methods (2018) IF: 2.073
A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

DOI: 10.1039/C8AY02113A

Sample: serum
Cancer Biomarkers (2016) IF: 1.736
Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis

DOI: 10.3233/CBM-150560

Sample: serum

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