|Detection range||3.91-250 ng/mL|
|Sample type &Sample volume||serum, plasma and other biological fluids; 100μL|
|Specificity||This kit recognizes Human DBP in samples. No significant cross-reactivity or interference between Human DBP and analogues was observed.|
|Reproducibility||Both intra-CV and inter-CV are < 10%.|
|Application||This ELISA kit applies to the in vitro quantitative determination of Human DBP concentrations in serum, plasma and other biological fluids.|
|Micro ELISA Plate(Dismountable)||96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
|-20℃, 6 months|
|Reference Standard||96T: 2 vials
48T: 1 vial
|Concentrated Biotinylated Detection Ab (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|Concentrated HRP Conjugate (100×)||96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
|-20℃(Protect from light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||2-8°C, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||2-8℃(Protect from light)|
|Stop Solution||1 vial, 10 mL||2-8°C|
|Plate Sealer||5 pieces|
|Certificate of Analysis||1 copy|
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human DBP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human DBP were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
The recovery of Human DBP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
|EDTA plasma (n=8)||89-103||96|
|Cell culture media (n=8)||86-99||92|
Samples were spiked with high concentrations of Human DBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results
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D*************aSubmitted [ Jan 25 2018 ]
Asked: Hello, Is kit running with monoclonal ab or policlonal ab ?
P*******nSubmitted [ Jan 25 2018 ]
Answered: Dear Dr. Gulsum Feyza, Thanks for your question. The kit is running with both monoclonal Ab and polyclonal Ab. The coated antibody is monoclonal and the detection antibody is polyclonal. Any other questions, please let us know. Or you could email us firstname.lastname@example.org