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Cat.No.:E-EL-H0149
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human IL-1β in samples. No significant cross-reactivity or interference between Human IL-1β and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human IL-1β concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-1β were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-1β were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 27.70 | 46.20 | 197.60 | 29.10 | 47.80 | 192.60 |
Standard deviation | 1.40 | 2.60 | 9.50 | 1.70 | 2.80 | 6.00 |
CV (%) | 5.05 | 5.63 | 4.81 | 5.84 | 5.86 | 3.12 |
The recovery of Human IL-1β spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-110 | 100 |
EDTA plasma (n=8) | 89-102 | 95 |
Cell culture media (n=8) | 84-98 | 91 |
Samples were spiked with high concentrations of Human IL-1β and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01584
|
Synonyms | IL1B, IL1-BETA, IL1F2, catabolin |
Research Area | Cancer, Cardiovascular, Metabolism, Immunology |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
|
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
|
3. Aspirate and wash the plate for 3 times |
|
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
|
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
|
6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 33015716 |
Sample: Cell culture medium |
DOI: 10.1002/hep.31056 |
|
PMID: 31808577 |
Sample: Serum |
DOI: 10.1111/jpi.12855 |
|
Sample: serum |
Sample: serum |
PMID: 35248068 |
Sample: serum |
PMID: 35768823 |
Sample: cerebrospinal fluid (CSF),peripheral monocytes |
PMID: 36155221 |
Sample: HMC3 cell |
PMID: 32755583 |
Sample: Plasma |
PMID: 33431796 |
Sample: Serum,Cell culture supernatant |
PMID: 34999169 |
Sample: serum,THP-1 cell,human circulating CD14+ monocytes |
Sample: NP cells |
PMID: 35145201 |
Sample: nucleus pulposus (NP) cell |
PMID: 35936005 |
Sample: RPE cell |
PMID: 33738142 |
Sample: Cell culture supernatant(HTR-8/SVneo cells,JEG-3 c |
PMID: 35863214 |
Sample: HK-2 cell |
Sample: HepG2 cell |
Sample: plasma,THP-1 cell |
PMID: 32241426 |
Sample: Gingival Crevicular Fluid |
PMID: 34875345 |
Sample: serum |
DOI: 10.1155/2022/3576892 |
|
PMID: 35855864 |
Sample: serum |
PMID: 36461199 |
Sample: Het-1A cell |
Sample: THP-1 monocytes |
Sample: Cell culture supernatant |
Sample: Human primary nucleus pulposus cell (HNPC) |
DOI: 10.3390/md21030156 |
|
Sample: HT1080 cell |
Sample: HCMEC/D3 cell,U87MG cell |
PMID: 31861116 |
Sample: Serum,Plasma,Whole Blood,Platelet |
PMID: 30448228 |
Sample: Cell culture supernatant |
Sample: THP-1 cells |
PMID: 36077562 |
Sample: THP-1 cell |
PMID: 34175826 |
Sample: hASMCs cells |
DOI: 10.1002/ptr.7628 |
|
PMID: 36199227 |
Sample: hVICs |
PMID: 33895109 |
Sample: Plasma |
PMID: 36145700 |
Sample: HaCaT cell |
DOI: 10.1155/2022/4235126 |
|
PMID: 35480873 |
Sample: NPCs |
DOI: 10.1155/2022/5249367 |
|
PMID: 35422896 |
Sample: Tregs |
DOI: 10.1155/2022/2776440 |
|
PMID: 35039758 |
Sample: nucleus pulposus cell (NPCs) |
DOI: 10.1155/2021/5584447 |
|
PMID: 34239691 |
Sample: NP cells |
Sample: Saliva |
Sample: serum |
Sample: NP cell |
PMID: 34239872 |
Sample: NP cells |
DOI: 10.3390/nu14183871 |
|
Sample: LO2 hepatocytes |
DOI: 10.3390/nu14142895 |
|
PMID: 35889856 |
Sample: serum |
DOI: 10.2147/JIR.S299010 |
|
Sample: ARPE-19 cell |
DOI: 10.2147/JIR.S361541 |
|
PMID: 35676970 |
Sample: Human corneal epithelial cell (HCECs) |
DOI: 10.2147/JIR.S317182 |
|
Sample: U937 cell |
DOI: 10.1002/JPER.21-0049 |
|
PMID: 34008185 |
Sample: serum |
Sample: BGC-823 cell,SGC-7901 cells |
PMID: 35007563 |
Sample: ARPE-19 cell |
DOI: 10.1111/cpr.12673 |
|
Sample: CaCO2 cells,HT‐29 cell |
Sample: blood |
Sample: Nucleus pulposus (NP) cell |
Sample: THP-1 cells |
DOI: 10.1039/D1FO03896F |
|
Sample: serum |
DOI: 10.1111/bph.13786 |
|
Sample: Cell culture supernatant |
Sample: serum |
Sample: plasma,cerebrospinal fluid |
PMID: 35551614 |
Sample: A549 cell |
Sample: Caco-2 cell |
Sample: HaCaT cells |
DOI: 10.1002/ptr.7113 |
|
PMID: 33899292 |
Sample: Serum |
PMID: 36091773 |
Sample: U87 cell |
PMID: 35873581 |
Sample: THP-1 cell |
Sample: MH7A cell |
DOI: 10.1111/ijpo.12872 |
|
PMID: 34881510 |
Sample: serum |
PMID: 34650715 |
Sample: serum |
PMID: 35461393 |
Sample: ARPE-19 cell |
DOI: 10.1002/tox.23464 |
|
PMID: 35006638 |
Sample: A549 cell |
DOI: 10.2147/DDDT.S325814 |
|
PMID: 34764638 |
Sample: Nthy-ori 3–1 cells |
PMID: 32063848 |
Sample: Cell culture supernatant |
PMID: 34819793 |
Sample: blood |
Sample: Serum,HK-2 cell |
PMID: 34237073 |
Sample: pleural fluid |
Sample: HUVECs cells |
DOI: 10.1002/cbin.12002 |
|
Sample: human retinal capillary endothelial cells (HRCECs) |
Sample: Cell culture supernatant |
PMID: 32145584 |
Sample: Cell culture supernatant |
PMID: 34459932 |
Sample: HREC |
PMID: 32585358 |
Sample: Cell culture supernatant |
DOI: 10.1155/2017/8519169 |
|
Sample: Cell culture supernatant |
Sample: Plasma |
PMID: 35429740 |
Sample: serum |
DOI: 10.1167/iovs.62.9.12 |
|
PMID: 34236384 |
Sample: HCECs cells |
DOI: 10.1155/2021/1125199 |
|
PMID: 34595242 |
Sample: serum |
DOI: 10.1155/2021/6695679 |
|
PMID: 33628853 |
Sample: Cell culture supernatant(HMECs) |
PMID: 32003754 |
Sample: Cell culture supernatant |
Sample: Cell culture medium |
Sample: THP-1 cell |
PMID: 35807509 |
Sample: human fibroblast cell |
PMID: 34794890 |
Sample: HGFs cells |
PMID: 33588175 |
Sample: Cell culture supernatant |
PMID: 35313270 |
Sample: Human gingival fibroblasts (HGFs) |
PMID: 34982483 |
Sample: THP-1 cell |
DOI: 10.1155/2019/6403504 |
|
Sample: rheumatoid arthritis (RA) fibroblast-like synovioc |
DOI: 10.1055/a-1878-5704 |
|
Sample: THP-1 cell |
Sample: Cell culture supernatant |
Sample: Saliva |
PMID: 32124161 |
Sample: Cell culture medium |
Sample: plasma |
Sample: rheumatoid arthritis (RA) fibroblast-like synovioc |
Sample: serum |
PMID: 30952009 |
Sample: Plasma,Cell culture supernatant,Bronchoalveolar La |
PMID: 30476824 |
Sample: Cell culture supernatant |
Sample: MH7A cells |
DOI: 10.1002/jcla.24579 |
|
PMID: 35819097 |
Sample: pleural fluid |
Sample: HEK293T cells |
PMID: 33009637 |
Sample: Cell culture supernatant |
DOI: 10.1155/2017/3605350 |
|
Sample: Serum |
PMID: 30550887 |
Sample: Cell culture supernatant |
Sample: BEAS-2B cell |
PMID: 34488537 |
Sample: A549 cell,H1299 cell |
PMID: 35006038 |
Sample: HaCaT cell |
PMID: 35510503 |
Sample: Human retinal microvascular endothelial cell (HREC |
PMID: 34898371 |
Sample: ARPE-19 cell |
Sample: THP-1 cells |
PMID: 31394160 |
Sample: Cell culture supernatant |
PMID: 35980503 |
Sample: THP-1 cell,H1299 cell |
Sample: Serum,Cell sample |
PMID: 33885954 |
Sample: Cell culture supernatant |
DOI: 10.5937/jomb0-33096 |
|
Sample: HK-2 cell |
DOI: 10.1155/2020/5341247 |
|
Sample: Serum |
PMID: 31683033 |
Sample: Cell culture supernatant |
PMID: 31783135 |
Sample: Cell culture supernatant |
Sample: sputum |
DOI: 10.1111/jgh.14961 |
|
PMID: 31860730 |
Sample: Serum,Tissue homogenate |
DOI: 10.1002/jcb.28724 |
|
Sample: rheumatoid arthritis (RA) fibroblast-like synovioc |
PMID: 34481822 |
Sample: piHCKs |
PMID: 35279426 |
Sample: cerebrospinal fluid (CSF) |
DOI: 10.1155/2018/9372436 |
|
Sample: serum |
Sample: SH-SY5Y cell |
PMID: 35986791 |
Sample: gingival crevicular fluid (GCF) |
DOI: 10.1002/jbt.22845 |
|
PMID: 34338401 |
Sample: ARPE-19 cell |
PMID: 34583676 |
Sample: serum,RA-FLS |
Sample: Cell culture supernatant |
PMID: 32497675 |
Sample: Serum,Cell culture supernatant |
PMID: 30844656 |
Sample: Wound drainage fluid |
PMID: 31440860 |
Sample: Serum |
DOI: 10.1042/BSR20191252 |
|
PMID: 31651935 |
Sample: Serum,Plasma,Cell sample |
Sample: Serum |
DOI: 10.21037/atm-20-7983 |
|
PMID: 33569461 |
Sample: HPMECs |
DOI: 10.21037/atm-21-6812 |
|
Sample: adipose-derived stem cell (ASCs) |
PMID: 33182037 |
Sample: Cell lysate |
PMID: 33217691 |
Sample: Cell culture supernatant |
PMID: 32502922 |
Sample: Cell culture supernatant |
DOI: 10.1111/jre.13087 |
|
Sample: Gingival crevicular fluid(GCF) |
Sample: Cell culture supernatant |
Sample: Saliva |
PMID: 32669704 |
Sample: Cell culture supernatant |
Sample: Plasma |
PMID: 33300058 |
Sample: Cell culture supernatant |
PMID: 34521466 |
Sample: blood |
PMID: 35579737 |
Sample: THP-1 monocytes |
DOI: 10.1002/jcla.23996 |
|
PMID: 34492129 |
Sample: serum |
Sample: Serum |
PMID: 34259387 |
Sample: THP-1 cell |
PMID: 31473895 |
Sample: Cell lysate |
Sample: NHBE cells |
Sample: A549 cell |
PMID: 33650318 |
Sample: Cell culture supernatant(THP-1) |
DOI: 10.1159/000513330 |
|
Sample: HCnEpCs |
PMID: 30528233 |
Sample: Serum |
DOI: 10.2147/NDT.S291164 |
|
PMID: 33727817 |
Sample: Serum |
PMID: 32669002 |
Sample: Serum |
PMID: 35412486 |
Sample: human normal colonic mucosal cells (FHCs) |
DOI: 10.1155/2021/9958808 |
|
Sample: THP-1 cells |
DOI: 10.1155/2022/7941039 |
|
PMID: 35378909 |
Sample: platelets suspensions |
Sample: Human Umbilical Vein Endothelial Cells (HUVECs) |
DOI: 10.1155/2022/2961187 |
|
PMID: 35281537 |
Sample: HK-2 cell |
DOI: 10.1039/C6FO00831C |
|
Sample: Plasma,Serum |
PMID: 35262594 |
Sample: gingival crevicular fluid (GCF) |
PMID: 31668807 |
Sample: Cell culture supernatant |
Sample: Saliva |
Sample: Cell culture supernatant,Serum |
PMID: 35866915 |
Sample: Ker-CT cell,HaCaT cell |
Sample: Human Umbilical Vein Endothelial Cells (HUVECs) |
DOI: 10.3967/bes2022.089 |
|
PMID: 35945185 |
Sample: THP-1 cell |
Sample: human histiocytic lymphoma (U-937) cell |
DOI: 10.3390/app13010485 |
|
Sample: plasma |
DOI: 10.1159/000518172 |
|
PMID: 34518457 |
Sample: HRGECs |
DOI: 10.1111/dth.14733 |
|
PMID: 33389789 |
Sample: Serum |
Sample: Cell culture supernatant |
PMID: 31838663 |
Sample: Cell culture supernatant |
Sample: serum |
PMID: 30688539 |
Sample: Synovia |
Sample: Cell culture supernatant |
Sample: serum |
PMID: 31938089 |
Sample: Beas2B cells |
Sample: Cell culture supernatant |
Sample: peripheral blood |
Sample: Serum |
PMID: 31629608 |
Sample: Serum |
PMID: 31394382 |
Sample: Cell culture supernatant |
Sample: Cell culture supernatant |
PMID: 33469482 |
Sample: Cell culture supernatant |
DOI: 10.12659/MSM.910740 |
|
Sample: Serum |
DOI: 10.12659/MSM.908592 |
|
Sample: Serum |
PMID: 32556004 |
Sample: Serum |
DOI: 10.12659/MSM.921957 |
|
PMID: 32463805 |
Sample: Cell culture supernatant |
PMID: 32805637 |
Sample: Cell culture supernatant |
DOI: 10.1242/bio.043653 |
|
Sample: L02 cells |
DOI: 10.1155/2019/5961742 |
|
PMID: 30941196 |
Sample: Cell culture supernatant |
DOI: 10.2152/jmi.69.180 |
|
Sample: follicular fluid,serum |
Sample: serum |