Human IL-17(Interleukin 17) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0105

      Synonyms:IL17

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human IL-17 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17. You can calculate the concentration of Human IL-17 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Human IL-17 in samples. No significant cross-reactivity or interference between Human IL-17 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      2000 2.355
      2.383
      2.369 2.291
      1000 1.557
      1.591
      1.574 1.496
      500 0.944
      0.918
      0.931 0.853
      250 0.472
      0.51
      0.491 0.413
      125 0.274
      0.27
      0.272 0.194
      62.5 0.182
      0.176
      0.179 0.101
      31.25 0.128
      0.132
      0.13 0.052
      0 0.074
      0.082
      0.078 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-17 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-17 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 95.20 215.10 996.10 94.90 200.40 990.40
      Standard deviation 6.30 10.10 37.90 6.00 9.20 50.50
      C V (%) 6.62 4.70 3.80 6.32 4.59 5.10

      Recovery

      The recovery of Human IL-17 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 87-99 93
      EDTA plasma (n=5) 85-97 90
      Cell culture media (n=5) 86-101 93

      Linearity

      Samples were spiked with high concentrations of Human IL-17 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 98-110 98-110 92-105
      Average (%) 104 104 98
      1:4 Range (%) 97-113 80-93 94-105
      Average (%) 105 87 99
      1:8 Range (%) 101-114 85-99 94-108
      Average (%) 108 90 101
      1:16 Range (%) 99-116 81-96 95-112
      Average (%) 106 88 103

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Publication: Wang Z Y, Jiang M J, Wu M H, et al. Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis[J]. International Journal of Clinical and Experimental Pathology, 2017, 10(3): 2887-2894.
        Species: Human
        Sample Type: Culture supernatant
      2. Publication: Morsy H, Taha E A, Nigm D A, et al. Serum IL-17 In Patients With Erythema Multiforme Or Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis Drug Reaction, And Correlation With Disease Severity[J]. Clinical and Experimental Dermatology, 2017.
        Species: Human
        Sample Type: Serum
      3. Publication: AL-Msaid H L F, AL-Sallami A S M. Study the Level of Cytokine in Unexplained and Idiopathic Infertile Men[J]. Journal of Pharmaceutical Sciences and Research, 2018, 10(4): 808-811.
        Species: Human
        Sample Type: Semen+serum
      4. Publication: Ruan G, Xu J, Wang K, et al. Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis[J]. Osteoarthritis and cartilage, 2018.
        Species: Human
        Sample Type: Serum
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