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Human PⅠCP(Procollagen Ⅰ C-Terminal Propeptide) ELISA Kit

Cat.No.:E-EL-H6030
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human PⅠCP in samples. No significant cross-reactivity or interference between Human PⅠCP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human PⅠCP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅠCP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅠCP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅠCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅠCP. You can calculate the concentration of Human PⅠCP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PⅠCP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PⅠCP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.34	5.03	22.39	2.15	6.09	21.50
Standard deviation	0.13	0.24	0.92	0.13	0.29	1.12
CV (%)	5.56	4.77	4.11	6.05	4.76	5.21

Recovery

The recovery of Human PⅠCP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-98	91
EDTA plasma (n=8)	93-104	100
Cell culture media (n=8)	96-102	99

Linearity

Samples were spiked with high concentrations of Human PⅠCP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	P1CP, C-Propeptide Of Type I Procollagen
Research Area	Signal Transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

DOI: 10.1016/j.biopha.2023.114289

Sample: cardiac fibroblast
Frontiers in Physiology (2020) IF: 3.367
Elevated β1-Adrenergic Receptor Autoantibody Levels Increase Atrial Fibrillation Susceptibility by Promoting Atrial Fibrosis

DOI: 10.3389/fphys.2020.00076

Sample: Serum
ESC Heart Failure (2022) IF: 3.612
Direct actions of dapagliflozin and interactions with LCZ696 and spironolactone on cardiac fibroblasts of patients with heart failure and reduced ejection fraction

DOI: 10.1002/ehf2.14186

PMID: 36303443
Sample: Cardiac fibroblasts
ANNALS OF THORACIC SURGERY (2020) IF: 3.639
Matrix Metalloproteinases Increase Because of Hypoperfusion in Obstructive Hypertrophic Cardiomyopathy

DOI: 10.1016/j.athoracsur.2020.05.156

Sample: plasma
Diagnostics (2022) IF: 3.706
The Role of Circulating Collagen Turnover Biomarkers and Late Gadolinium Enhancement in Patients with Non-Ischemic Dilated Cardiomyopathy

DOI: 10.3390/diagnostics12061435

PMID: 35741245
Sample: serum
HEART AND VESSELS (2015) IF: 2.065
Extracellular matrix turnover in coronary artery ectasia patients

DOI: 10.1007/s00380-014-0622-4

Sample: Serum
BMC Cardiovascular Disorders (2021) IF: 2.298
Increased ratio of sST2/LVMI predicted cardiovascular mortality and heart failure rehospitalization in heart failure with reduced ejection fraction patients: a prospective cohort study

DOI: 10.1186/s12872-021-02191-3

PMID: 34404341
Sample: serum
CARDIOVASCULAR PATHOLOGY (2019) IF: 1.765
Procollagen type I carboxy-terminal propeptide (PICP) and MMP-2 are potential biomarkers of myocardial fibrosis in patients with hypertrophic cardiomyopathy

DOI: 10.1016/j.carpath.2019.107150

Sample: plasma

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Reviews/Q&A

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Reviews
Q&A

D***************oSubmitted [ Feb 16 2023 ]

Asked: What is the normal range for PICP in myocardial fibrosis with this kit? From another study (not elabscience kit), the range is above 100 ng/ml, but in this kit i got the result below 40 ng/ml. can i have explanation?

adminSubmitted [ Feb 16 2023 ]

Answered: Hi, Destrian.Thanks for your feedback. According to the available references, the PICP content level of normal human serum plasma is about 20 ng/ml. The detected results of our ELISA Kit were consistent with those reported in the references. However, due to the differences of individuals and different brands of ELISA Kit, the samples may have low or high values. In addition, dilution factor may also be one of the factors contributing to the low values. Therefore, it is suggested that you provide the lot number of ELISA Kit, sample type and the raw data of ELISA Kit, so that we can help you solve your problem better.

G******************iSubmitted [ Nov 10 2022 ]

Asked: May i get the normal range of PICP for this kit? I have used this kit for my research and found that the result was much lower than most publication (range >100 ng/ml). Thank you.

adminSubmitted [ Nov 10 2022 ]

Answered: Hi, Giovanni Gerry Tampi. I'll email to you later.

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