Human TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-H0110

      Synonyms:TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1

      • 96T
      • 24T
      - +
      Price: $495

      Reactivity: Human

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Human TGF-β1 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-β1. You can calculate the concentration of Human TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Human
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Human TGF-β1 in samples. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      2000 2.378
      2.381 2.323
      1000 1.635
      1.653 1.595
      500 0.965
      0.955 0.897
      250 0.471
      0.479 0.421
      125 0.252
      0.247 0.189
      62.5 0.161
      0.159 0.101
      31.25 0.104
      0.11 0.052
      0 0.054
      0.058 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TGF-β1 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TGF-β1 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 106.20 233.50 976.30 109.60 234.10 999.00
      Standard deviation 7.20 11.90 51.70 5.60 12.40 43.00
      C V (%) 6.78 5.10 5.30 5.11 5.30 4.30


      The recovery of Human TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 94-105 100
      EDTA plasma (n=5) 90-104 96
      Cell culture media (n=5) 87-100 92


      Samples were spiked with high concentrations of Human TGF-β1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 85-100 97-111 94-111
      Average (%) 91 102 101
      1:4 Range (%) 92-108 83-96 90-108
      Average (%) 100 89 98
      1:8 Range (%) 96-108 81-96 92-107
      Average (%) 102 88 98
      1:16 Range (%) 98-117 79-92 95-108
      Average (%) 107 86 102

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      2.Remove the liquid.

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Zhang L, Wang Y, Xia T, et al. Suppression For Lung Metastasis By Depletion Of Collagen I And Lysyl Oxidase Via Losartan Assisted With Paclitaxel-Loaded Ph-Sensitive Liposomes In Breast Cancer[J]. Drug Delivery, 2016.
        Sample Type: Cell lysis
      2. Publication: Zhang J G, Chen X J, Liu T, et al. Foxp3+ Associated With The Pro-Inflammatory Regulatory T And T Helper 17 Effector Cells In Asthma Patients[J]. Experimental and Therapeutic Medicine, 2016, 12(4): 2753-2758.
        Sample Type: Plasma
      3. Publication: Liu Y, Li Y, Xu Q, et al. Long Non-Coding RNA-ATB Promotes EMT During Silica-Induced Pulmonary Fibrosis By Competitively Binding miR-200c[J]. Biochimica Et Biophysica Acta-Molecular Basis of Disease, 2017.
        Sample Type: culture supernatant
      4. Publication: Giusti I, Di Francesco M D, D'Ascenzo S, et al. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma[J]. Experimental and Therapeutic Medicine, 2018, 15(4): 4029-4038.
      5. Publication: Fan X, Teng Y, Ye Z, et al. Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs[J]. J Cell Sci, 2018: jcs. 216135.
        Sample Type: Rat
      6. Publication: Thu H E, Hussain Z, Mohamed I N, et al. Mechanistic Insight Into the Efficient Osteogenic Potential of Dihydrotestosterone: Exploring Sequential Expression of Bone-Related Protein Biomarkers[J]. Acta Medica Iranica, 2018, 56(4): 234-243.
        Sample Type: Human
      7. Publication: Gu T T , Chen T Y , Yang Y Z , et al. Pterostilbene alleviates fructose-induced renal fibrosis by suppressing TGF-β1/TGF-β type I receptor/Smads signaling in proximal tubular epithelial cells[J]. European Journal of Pharmacology, 2018.
        Sample Type: Serum,Cell supernatants
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