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LLC-MK2 cell line

  • Cat.No.:EP-CL-0141

To Purchase EP-CL-0141

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  • 1×10⁶ cells/vial
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Origin and General Characteristics

Cell Name LLC-MK2 cell line
Organism Macaca mulatta, Monkey, Rhesus
Age adult
Tissue Kidney
Morphology Epithelial
Growth Properties Adherent
Descriptions This cell line was established in the 1950s from a renal tissue pooled suspension from six rhesus monkeys (Macaca mulatta). The cells are reported susceptible to a wide range of viruses including members of the enterovirus, rhinovirus, mucinous and poxvirus groups.
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium RPMI-1640+10% FBS+1% P/S
(LLC-MK2 cell line complete medium (EP-ML-0141) is recommended.)
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1: 3-1: 4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

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