Origin and General Characteristics
||LLC-MK2 cell line
||Macaca mulatta, Monkey, Rhesus
||This cell line was established in the 1950s from a renal tissue pooled suspension from six rhesus monkeys (Macaca mulatta). The cells are reported susceptible to a wide range of viruses including members of the enterovirus, rhinovirus, mucinous and poxvirus groups.
Culture Conditions and Handling
|Complete Growth Medium
||RPMI-1640+10% FBS+1% P/S
||Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.
Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
||1: 3-1: 4
||Every 2 to 3 days
||Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
||Atmosphere: Air, 95%; CO2, 5%
Special Features of the Cell Line
Recommendations for handling of cryopreserved cells