LLC-MK2 cell line

Name: LLC-MK2 cell line
Brand: Elabscience
SKU: EP-CL-0141
Availability: InStock

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Cell Name	LLC-MK2 cell line
Organism	Macaca mulatta, Monkey, Rhesus
Age	adult
Tissue	Kidney
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	This cell line was established in the 1950s from a renal tissue pooled suspension from six rhesus monkeys (Macaca mulatta). The cells are reported susceptible to a wide range of viruses including members of the enterovirus, rhinovirus, mucinous and poxvirus groups.
Biosafety Level	1

Complete Growth Medium	RPMI-1640+10% FBS+1% P/S (LLC-MK2 cell line complete medium (EP-ML-0141) is recommended.)
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1: 3-1: 4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

EP-CM-P0139	MEM (with NEAA), powder
EP-CM-L0048	Penicillin-Streptomycin Solution (100×)
EP-CM-L0006	Phosphate Buffer (PBS)
EP-CM-L0060	200mM L-Alanyl-L-Glutamine Solution (100×)
EP-CM-L0043	0.25% Trypsin Solution

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