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Cat.No.:EP-CL-0141
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Add to cart Bulk requestCell Name | LLC-MK2 cell line |
Organism | Macaca mulatta, Monkey, Rhesus |
Age | adult |
Tissue | Kidney |
Morphology | Epithelial |
Growth Properties | Adherent |
Descriptions | This cell line was established in the 1950s from a renal tissue pooled suspension from six rhesus monkeys (Macaca mulatta). The cells are reported susceptible to a wide range of viruses including members of the enterovirus, rhinovirus, mucinous and poxvirus groups. |
Biosafety Level | 1 |
Complete Growth Medium | RPMI-1640+10% FBS+1% P/S (LLC-MK2 cell line complete medium (EP-ML-0141) is recommended.) |
Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
Subcultivation Ratio | 1: 3-1: 4 |
Medium Renewal | Every 2 to 3 days |
Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Recommendations for handling of cryopreserved cells
Protocol for Thawing Frozen Cells
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