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Mouse CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

Citations(44)

  • Cat.No.:E-EL-M3023

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse CTXⅠ in samples. No significant cross-reactivity or interference between Mouse CTXⅠ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse CTXⅠ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CTXⅠ. You can calculate the concentration of Mouse CTXⅠ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CTXⅠ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CTXⅠ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 82.17 221.85 948.92 87.03 203.37 803.81
Standard deviation 4.48 10.85 48.30 4.99 9.56 33.28
CV (%) 5.45 4.89 5.09 5.73 4.70 4.14

Recovery

The recovery of Mouse CTXⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-98 91
EDTA plasma (n=8) 86-96 91
Cell culture media (n=8) 94-108 101

Linearity

Samples were spiked with high concentrations of Mouse CTXⅠ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCTX-I, CTX1
Research AreaMetabolism, Cardiovascular, Cancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Advanced Science (2021) IF: 16.806
    Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y

    DOI: 10.1002/advs.202100808

    PMID: 34719888

    		 Sample: serum
  2. Advanced Science (2021) IF: 16.806
    Extracellular Vesicles from Child Gut Microbiota Enter into Bone to Preserve Bone Mass and Strength

    DOI: 10.1002/advs.202004831

    PMID: 33977075

    		 Sample: Serum
  3. CELL DEATH AND DIFFERENTIATION (2021) IF: 15.828
    Site-1 protease controls osteoclastogenesis by mediating LC3 transcription

    DOI: 10.1038/s41418-020-00731-6

    PMID: 33469231

    		 Sample: Serum
  4. EMBO Molecular Medicine (2022) IF: 14.26
    Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2

    DOI: 10.15252/emmm.202115373

    PMID: 35611810

    		 Sample: serum
  5. Nature Communications (2022) IF: 14.919
    Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox

    DOI: 10.1038/s41467-022-29191-x

    PMID: 35304471

    		 Sample: Serum
  6. DEVELOPMENTAL CELL (2022) IF: 13.417
    Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption

    DOI: 10.1016/j.devcel.2022.09.013

    PMID: 36243012

    		 Sample: serum,osteoclasts
  7. Bone Research (2019) IF: 13.567
    Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG

    DOI: 10.1038/s41413-019-0056-9
    Sample: serum
  8. CELL DEATH AND DIFFERENTIATION (2022) IF: 12.067
    HSC70 mediated autophagic degradation of oxidized PRL2 is responsible for osteoclastogenesis and inflammatory bone destruction

    DOI: 10.1038/s41418-022-01068-y

    PMID: 36182990

    		 Sample: serum
  9. Bone Research (2018) IF: 12.354
    Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines

    DOI: 10.1038/s41413-018-0012-0
    Sample: Serum
  10. Redox Biology (2021) IF: 11.799
    CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners

    DOI: 10.1016/j.redox.2021.102180

    PMID: 34763297

    		 Sample: plasma
  11. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    Cladrin alleviates dexamethasone-induced apoptosis of osteoblasts and promotes bone formation through autophagy induction via AMPK/mTOR signaling

    DOI: 10.1016/j.freeradbiomed.2022.08.028

    PMID: 35998794

    		 Sample: serum
  12. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    Chebulinic acid alleviates LPS-induced inflammatory bone loss by targeting the crosstalk between reactive oxygen species/NFκB signaling in osteoblast cells

    DOI: 10.1016/j.freeradbiomed.2022.11.026
    Sample: serum
  13. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/β-catenin mediated osteogenesis

    DOI: 10.1016/j.freeradbiomed.2022.08.007

    PMID: 35963563

    		 Sample: serum
  14. Cell Reports (2020) IF: 8.109
    SENP3 Suppresses Osteoclastogenesis by De-conjugating SUMO2/3 from IRF8 in Bone Marrow-Derived Monocytes

    DOI: 10.1016/j.celrep.2020.01.036
    Sample: serum
  15. CELL DEATH AND DIFFERENTIATION (2016) IF: 8.218
    IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4 + T cells

    DOI: 10.1038/cdd.2016.72
    Sample: Serum
  16. Theranostics (2020) IF: 8.5790
    IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    DOI: 10.7150/thno.41378
    Sample: serum
  17. Theranostics (2020) IF: 8.5790
    Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism

    DOI: 10.7150/thno.39238
    Sample: serum
  18. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245
    Sample: plasma
  19. JOURNAL OF CELLULAR PHYSIOLOGY (2021) IF: 6.384
    POLR2A blocks osteoclastic bone resorption and protects against osteoporosis by interacting with CREB1

    DOI: 10.1002/jcp.30220

    PMID: 33595106

    		 Sample: Serum
  20. CELLULAR AND MOLECULAR LIFE SCIENCES (2020) IF: 6.496
    Maternal high protein-diet programs impairment of offspring’s bone mass through miR-24-1-5p mediated targeting of SMAD5 in osteoblasts

    DOI: 10.1007/s00018-020-03608-6
    Sample: Serum
  21. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Wheel-Running Exercise Protects Ovariectomized Mice from Bone Loss via IFN-γ-Mediated Suppression of the NF-κB and MAPK Pathways

    DOI: 10.1155/2022/2030818
    Sample: serum
  22. International Journal of Biological Sciences (2022) IF: 6.582
    Myokine Irisin promotes osteogenesis by activating BMP/SMAD signaling via αV integrin and regulates bone mass in mice

    DOI: 10.7150/ijbs.63505

    PMID: 35002510

    		 Sample: serum
  23. Nutrients (2022) IF: 6.706
    Peptide−Calcium Chelate from Antler (Cervus elaphus) Bone Enhances Calcium Absorption in Intestinal Caco-2 Cells and D-gal-Induced Aging Mouse Model

    DOI: 10.3390/nu14183738
    Sample: serum
  24. CELL PROLIFERATION (2022) IF: 6.831
    Urolithin B suppressed osteoclast activation and reduced bone loss of osteoporosis via inhibiting ERK/NF-κB pathway

    DOI: 10.1111/cpr.13291

    PMID: 35708050

    		 Sample: serum
  25. CELL PROLIFERATION (2021) IF: 6.831
    Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

    DOI: 10.1111/cpr.13087

    PMID: 34255393

    		 Sample: 3T3 cells
  26. Biomaterials Science (2021) IF: 6.843
    Irisin recouples osteogenesis and osteoclastogenesis to protect wear-particle-induced osteolysis by suppressing oxidative stress and RANKL production

    DOI: 10.1039/D1BM00563D

    PMID: 34323888

    		 Sample: sera
  27. Bone & Joint Research (2022) IF: 5.853
    N,N-Dimethylformamide inhibits high glucose-induced osteoporosis via attenuating MAPK and NF-κB signalling

    DOI: 10.1302/2046-3758.114.BJR-2020-0308.R2
    Sample: plasma
  28. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice

    DOI: 10.3390/ijms22179590

    PMID: 34502499

    		 Sample: plasma
  29. CELLULAR SIGNALLING (2021) IF: 4.315
    Osteosarcoma-derived exosomal miR-501-3p promotes osteoclastogenesis and aggravates bone loss

    DOI: 10.1016/j.cellsig.2021.109935

    PMID: 33529755

    		 Sample: Serum
  30. CALCIFIED TISSUE INTERNATIONAL (2021) IF: 4.333
    Phenanthrenoid Coelogin Isolated from Coelogyne cristata Exerts Osteoprotective Effect Through MAPK-Mitogen-Activated Protein Kinase Signaling Pathway

    DOI: 10.1007/s00223-021-00818-3

    PMID: 33675370

    		 Sample: Serum
  31. Human Cell (2022) IF: 4.374
    METTL14 upregulates TCF1 through m6A mRNA methylation to stimulate osteogenic activity in osteoporosis

    DOI: 10.1007/s13577-022-00825-y
    Sample: serum
  32. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-487b-3p impairs osteoblastogenesis by targeting Notch-regulated ankyrin-repeat protein (Nrarp)

    DOI: 10.1530/JOE-19-0015

    PMID: 30978699

    		 Sample: Serum
  33. BONE (2021) IF: 4.398
    Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

    DOI: 10.1016/j.bone.2021.116211

    PMID: 34560308

    		 Sample: serum
  34. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
    HDAC inhibitor quisinostat prevents estrogen deficiency-induced bone loss by suppressing bone resorption and promoting bone formation in mice

    DOI: 10.1016/j.ejphar.2022.175073

    PMID: 35636521

    		 Sample: serum
  35. JOURNAL OF CELLULAR BIOCHEMISTRY (2022) IF: 4.48
    Maintenance of increased bone mass after PTH withdrawal by sequential medicarpin treatment via augmentation of cAMP-PKA pathway

    DOI: 10.1002/jcb.30313

    PMID: 35959633

    		 Sample: serum
  36. JOURNAL OF CELLULAR PHYSIOLOGY (2019) IF: 4.522
    miR‐409‐5p negatively regulates Wnt/Beta catenin signaling pathway by targeting Lrp‐8

    DOI: 10.1002/jcp.28919
    Sample: serum
  37. Disease Models & Mechanisms (2020) IF: 4.651
    Akkermansia muciniphila promotes type H vessels formation and bone fracture healing by reducing gut permeability and inflammation

    DOI: 10.1242/dmm.043620
    Sample: serum
  38. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    Epoxymicheliolide inhibits osteoclastogenesis and resists OVX-induced osteoporosis by suppressing ERK1/2 and NFATc1 signaling

    DOI: 10.1016/j.intimp.2022.108632

    PMID: 35248947

    		 Sample: serum
  39. JOURNAL OF MOLECULAR MEDICINE-JMM (2018) IF: 4.938
    Identification of novel microRNA inhibiting actin cytoskeletal rearrangement thereby suppressing osteoblast differentiation

    DOI: 10.1007/s00109-018-1624-y
    Sample: Serum
  40. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Beneficial effects of hyperoside on bone metabolism in ovariectomized mice

    DOI: 10.1016/j.biopha.2018.08.069
    Sample: serum
  41. ENDOCRINE (2015) IF: 3.878
    Preservation of high-fat diet-induced femoral trabecular bone loss through genetic target of TNF-α

    DOI: 10.1007/s12020-015-0554-5
    Sample: Serum
  42. BIOELECTROMAGNETICS (2021) IF: 2.01
    Effects of Pulsed Electromagnetic Field Therapy at Different Frequencies on Bone Mass and Microarchitecture in Osteoporotic Mice

    DOI: 10.1002/bem.22344

    PMID: 34082467

    		 Sample: serum
  43. ACTA HISTOCHEMICA (2022) IF: 2.147
    TGFβ1-modified MSC-derived exosome attenuates osteoarthritis by inhibiting PDGF-BB secretion and H-type vessel activity in the subchondral bone

    DOI: 10.1016/j.acthis.2022.151933

    PMID: 35933783

    		 Sample: bone marrow
  44. JOURNAL OF BONE AND MINERAL METABOLISM (2021) IF: 2.626
    Protective effects of berberine on senile osteoporosis in mice

    DOI: 10.1007/s00774-021-01225-2

    PMID: 33829324

    		 Sample: Serum
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