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Mouse IgE(Immunoglobulin E) ELISA Kit

Citations(14)

Cat.No.:E-EL-M3034
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	125.00-8000 ng/mL
Sensitivity	75.00 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IgE in samples. No significant cross-reactivity or interference between Mouse IgE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IgE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IgE. You can calculate the concentration of Mouse IgE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IgE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IgE were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	382.47	858.80	3319.11	351.27	946.20	3588.32
Standard deviation	21.34	40.54	153.34	19.81	46.65	191.98
CV (%)	5.58	4.72	4.62	5.64	4.93	5.35

Recovery

The recovery of Mouse IgE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	101-111	104
EDTA plasma (n=8)	86-97	93
Cell culture media (n=8)	94-108	102

Linearity

Samples were spiked with high concentrations of Mouse IgE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	IgE
Research Area	Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

MACROMOLECULAR BIOSCIENCE (2022) IF: 5.859

Enhanced Transdermal Absorption of Hyaluronic Acid via Fusion with Pep-1 and A Hyaluronic Acid Binding Peptide

DOI: 10.1002/mabi.202200173
	Sample: Serum

FASEB JOURNAL (2021) IF: 5.192

Enhanced phenotype of calcipotriol-induced atopic dermatitis in filaggrin-deficient mice

DOI: 10.1096/fj.202002709R
PMID: 33855746	Sample: Serum

BIOLOGICAL RESEARCH (2019) IF: 2.482

Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice

DOI: 10.1186/s40659-019-0216-9
PMID: 30823938	Sample: Plasma

PHARMACOLOGY (2023) IF: 3.429

DPP4 Inhibitor Sitagliptin Reduces Inflammatory Responses and Mast Cell Activation in Allergic Rhinitis

DOI: 10.1159/000528634
	Sample: Serum

ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY (2016) IF: 3.475

Effect of treatment with geraniol on ovalbumin-induced allergic asthma in mice

DOI: 10.1016/j.anai.2016.03.029
	Sample: Serum

Parasites & Vectors (2021) IF: 3.876

In vivo and in vitro efficacy of crocin against Echinococcus multilocularis

DOI: 10.1186/s13071-021-04866-4
PMID: 34256821	Sample: Serum

Parasites & Vectors (2021) IF: 3.876

In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

DOI: 10.1186/s13071-021-04952-7
PMID: 34488852	Sample: Serum

JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018

Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

DOI: 10.1016/j.jri.2020.103240
PMID: 33166807	Sample: Serum

Scientific Reports (2017) IF: 4.259

Rheb1 deletion in myeloid cells aggravates OVA-induced allergic inflammation in mice

DOI: 10.1038/srep42655
	Sample: Bronchoalveolar Lavage Fluid,Serum

JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36

Immunomodulatory and anti-inflammatory effects of Aerva lanata in ovalbumin induced allergic asthmatic mice

DOI: 10.1016/j.jep.2022.115087
PMID: 35143934	Sample: Lung

Food & Function (2021) IF: 5.396

Reducing the allergenicity of pea protein based on the enzyme action of alcalase

DOI: 10.1039/D1FO00083G
PMID: 34031673	Sample: Serum

INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714

Phellopterin alleviates atopic dermatitis-like inflammation and suppresses IL-4-induced STAT3 activation in keratinocytes

DOI: 10.1016/j.intimp.2022.109270
PMID: 36179418	Sample: Serum

BRITISH JOURNAL OF PHARMACOLOGY (2022) IF: 9.473

Scutellarein Attenuates Atopic Dermatitis by Selectively Inhibiting Transient Receptor Potential Vanilloid 3

DOI: 10.1111/bph.15913
	Sample: Serum

SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753

Aerofloral studies and allergenicity of dominant pollen types in Taraba and Bauchi States of Northeastern Nigeria

DOI: 10.1016/j.scitotenv.2022.157076
PMID: 35780899	Sample: Plasma

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Reviews/Q&A

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Reviews
Q&A

M***********dSubmitted [ Aug 28 2023 ]

Asked: What dilution should be taken for serum samples?

adminSubmitted [ Aug 28 2023 ]

Answered: For E-EL-M3034 kit, it is recommended to dilute serum samples at 1-10 folds to test.

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