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Mouse MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Citations(48)Uniprot : P10148

  • Cat.No.:E-EL-M3001

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MCP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 94.05 210.98 994.91 88.91 244.77 840.43
Standard deviation 5.14 10.06 47.86 4.82 12.09 45.97
CV (%) 5.47 4.77 4.81 5.42 4.94 5.47

Recovery

The recovery of Mouse MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-104 100
EDTA plasma (n=8) 87-99 92
Cell culture media (n=8) 90-100 96

Linearity

Samples were spiked with high concentrations of Mouse MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P10148
SynonymsCCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Bioactive Materials (2021) IF: 14.593
    Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

    DOI: 10.1016/j.bioactmat.2021.07.019

    PMID: 34820587

    		 Sample: B16–F10 cells,Red blood cells
  2. ACS Applied Materials & Interfaces (2022) IF: 10.383
    Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment

    DOI: 10.1021/acsami.2c14448

    PMID: 36314479

    		 Sample: Hepa 1-6 cells-xenograft
  3. CARBOHYDRATE POLYMERS (2022) IF: 10.723
    Aloe polymeric acemannan inhibits the cytokine storm in mouse pneumonia models by modulating macrophage metabolism

    DOI: 10.1016/j.carbpol.2022.120032

    PMID: 36184177

    		 Sample: bronchoalveolar fluid,RAW264.7 cell
  4. Cell Death & Disease (2020) IF: 8.4689
    MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy

    DOI: 10.1038/s41419-020-02857-4
    Sample: BALF
  5. International Journal of Nanomedicine (2022) IF: 7.033
    Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice

    DOI: 10.2147/IJN.S384906
    Sample: serum
  6. Oxidative Medicine and Cellular Longevity (2023) IF: 7.31
    RRP Regulates Autophagy through the AMPK Pathway to Alleviate the Effect of Cell Senescence on Atherosclerosis

    DOI: 10.1155/2023/9645789
    Sample: serum
  7. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1α-SLUG Axis in Acute Respiratory Distress Syndrome

    DOI: 10.1155/2022/1030238
    Sample: serum,Human pulmonary alveolar epithelial cells (H
  8. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589
    Sample: plasma
  9. FREE RADICAL BIOLOGY AND MEDICINE (2020) IF: 6.17
    Hepatocyte-specific deficiency of Nrf2 exacerbates carbon tetrachloride-induced liver fibrosis via aggravated hepatocyte injury and subsequent inflammatory and fibrogenic responses

    DOI: 10.1016/j.freeradbiomed.2020.02.015

    PMID: 32112813

    		 Sample: Primary hepatocytes
  10. Food & Function (2022) IF: 6.317
    Serum metabolomics analysis reveals amelioration effects of sea cucumber ether phospholipids on oxidative stress and inflammation in high-fat diet-fed mice

    DOI: 10.1039/D2FO00918H

    PMID: 36106708

    		 Sample: serum
  11. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

    DOI: 10.1016/j.biopha.2021.112133

    PMID: 34474337

    		 Sample: serum
  12. Oxidative Medicine and Cellular Longevity (2016) IF: 6.543
    Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice

    DOI: 10.1155/2016/2715718
    Sample: Plasma
  13. LIFE SCIENCES (2020) IF: 5.037
    Fenugreek attenuates obesity-induced inflammation and improves insulin resistance through downregulation of iRhom2/TACE

    DOI: 10.1016/j.lfs.2020.118222

    PMID: 32768577

    		 Sample: Serum
  14. Oncotarget (2017) IF: 5.168
    Wu-tou decoction attenuates neuropathic pain via suppressing spinal astrocytic IL-1R1/TRAF6/JNK signaling

    DOI: 10.18632/oncotarget.21638
    Sample: Tissue homogenate
  15. Oncotarget (2017) IF: 5.168
    AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema

    DOI: 10.18632/oncotarget.15116
    Sample: Bronchoalveolar Lavage Fluid
  16. FASEB JOURNAL (2021) IF: 5.192
    Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

    DOI: 10.1096/fj.202101030R

    PMID: 34516679

    		 Sample: plasma
  17. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.279
    Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice

    DOI: 10.1021/acs.jafc.1c07482

    PMID: 35232013

    		 Sample: Serum
  18. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
    Anti-IFN-γ Antibody Promotes Osteoclastogenesis in Human Bone Marrow Monocyte-Derived Macrophages Co-Cultured with Tuberculosis-Activated Th1 Cells

    DOI: 10.1159/000493455
    Sample: CD4+T helper 1 cells(Th1 cells),bone marrow monocy
  19. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice

    DOI: 10.1016/j.intimp.2022.109092

    PMID: 35940075

    		 Sample: plasma
  20. Nutrients (2021) IF: 5.719
    Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice

    DOI: 10.3390/nu13093209

    PMID: 34579088

    		 Sample: serum
  21. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
    Anti-Obesity and Anti-Hyperglycemic Effects of Meretrix lusoria Protamex Hydrolysate in ob/ob Mice

    DOI: 10.3390/ijms23074015

    PMID: 35409375

    		 Sample: serum
  22. INFLAMMATION (2022) IF: 4.092
    Mesenchymal Stem Cells Attenuate Acute Lung Injury in Mice Partly by Suppressing Alveolar Macrophage Activation in a PGE2-Dependent Manner

    DOI: 10.1007/s10753-022-01670-9

    PMID: 35699823

    		 Sample: MH-S cell
  23. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Limonin ameliorates acute pancreatitis by suppressing JAK2/STAT3 signaling pathway

    DOI: 10.1002/tox.23352

    PMID: 34423886

    		 Sample: serum
  24. FEBS LETTERS (2022) IF: 4.124
    The Microglial membrane receptor TREM2 mediates exosome secretion to promote phagocytosis of amyloid-β by microglia

    DOI: 10.1002/1873-3468.14336

    PMID: 35292963

    		 Sample: Bv2 cell
  25. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
    Changes in Allergenicity of Ovalbumin in Vitro and in Vivo on Conjugation with Quercetin

    DOI: 10.1021/acs.jafc.0c00461

    PMID: 32182051

    		 Sample: Serum,Cell culture supernatant
  26. CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2021) IF: 4.33
    The role of Dock2 on macrophage migration and functions during Citrobacter rodentium infection

    DOI: 10.1111/cei.13590

    PMID: 33662140

    		 Sample: Colon tissue
  27. MOLECULES (2021) IF: 4.412
    Rosmanol and Carnosol Synergistically Alleviate Rheumatoid Arthritis through Inhibiting TLR4/NF-κB/MAPK Pathway

    DOI: 10.3390/molecules27010078
    Sample: serum
  28. NEUROPHARMACOLOGY (2020) IF: 4.431
    Seizure-induced neuroinflammation contributes to ectopic neurogenesis and aggressive behavior in pilocarpine-induced status epilepticus mice

    DOI: 10.1016/j.neuropharm.2020.108044

    PMID: 32179291

    		 Sample: Cell lysate
  29. Journal of Functional Foods (2021) IF: 4.451
    N-acetyl-chitooligosaccharide attenuates inflammatory responses by suppression of NF-κB signaling, MAPK and NLRP3 inflammasome in macrophages

    DOI: 10.1016/j.jff.2021.104364
    Sample: Cell culture medium
  30. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

    DOI: 10.1016/j.biopha.2020.110684
    Sample: serum
  31. MEDIATORS OF INFLAMMATION (2021) IF: 4.711
    The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice

    DOI: 10.1155/2021/7372893

    PMID: 33551679

    		 Sample: Serum
  32. Journal of Immunology Research (2021) IF: 4.818
    Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis

    DOI: 10.1155/2021/9409777

    PMID: 34812410

    		 Sample: U87 cells
  33. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 4.932
    COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice

    DOI: 10.1016/j.intimp.2020.106801
    Sample: Tissue homogenate
  34. JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021) IF: 3.011
    Rhamnetin ameliorates macrophage-mediated inflammation and pro-atherosclerosis pathways in apolipoprotein E-deficient mice

    DOI: 10.26402/jpp.2021.2.10
    Sample: aorta
  35. ACTA PHARMACOLOGICA SINICA (2017) IF: 3.223
    Molecular mechanisms of the analgesic action of Wu-tou Decoction on neuropathic pain in mice revealed using microarray and network analysis

    DOI: 10.1038/aps.2017.110
    Sample: Tissue homogenate
  36. Cardiovascular Toxicology (2021) IF: 3.239
    Diet-Derived Advanced Glycation End Products (dAGEs) Induce Proinflammatory Cytokine Expression in Cardiac and Renal Tissues of Experimental Mice: Protective Effect of Curcumin

    DOI: 10.1007/s12012-021-09697-4

    PMID: 34655413

    		 Sample: serum
  37. Bioengineered (2021) IF: 3.269
    The protective effects of Pimavanserin against cerebral ischemia-induced brain injury

    DOI: 10.1080/21655979.2021.1978617

    PMID: 34605354

    		 Sample: brain tissue
  38. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A (2015) IF: 3.369
    Dendritic cells enhance UHMWPE wear particle‐induced osteoclast differentiation of macrophages

    DOI: 10.1002/jbm.a.35459
    Sample: Cell culture supernatant
  39. BMC IMMUNOLOGY (2022) IF: 3.594
    NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

    DOI: 10.1186/s12865-022-00515-2

    PMID: 35965334

    		 Sample: Raw 264.7 cell
  40. TOXICOLOGICAL SCIENCES (2020) IF: 3.703
    Effect of Intratracheal Instillation of ZnO Nanoparticles on Acute Lung Inflammation Induced by Lipopolysaccharides in Mice

    DOI: 10.1093/toxsci/kfz234
    Sample: BALF
  41. BRAIN RESEARCH BULLETIN (2023) IF: 3.715
    IGF2 inhibits hippocampal over-activated microglia and alleviates depression-like behavior in LPS- treated male mice

    DOI: 10.1016/j.brainresbull.2023.01.001
    Sample: Serum
  42. CYTOKINE (2022) IF: 3.861
    IL-17 upregulates MCP-1 expression via Act1 / TRAF6 / TAK1 in experimental autoimmune myocarditis

    DOI: 10.1016/j.cyto.2022.155823

    PMID: 35183823

    		 Sample: serum
  43. INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943
    Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration

    DOI: 10.1016/j.intimp.2020.106190

    PMID: 31945611

    		 Sample: Cell culture supernatant
  44. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Bee venom attenuates neurodegeneration and motor impairment and modulates the response to L-dopa or rasagiline in a mice model of Parkinson’s disease

    DOI: 10.22038/ijbms.2020.46469.10731
    Sample: Tissue homogenate
  45. PLoS One (2017) IF: 2.806
    Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet

    DOI: 10.1371/journal.pone.0185688
    Sample: Serum
  46. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    4-Hydroxyisoleucine relieves inflammation through iRhom2-dependent pathway in co-cultured macrophages and adipocytes with LPS stimulation

    DOI: 10.1186/s12906-020-03166-1

    PMID: 33298044

    		 Sample: Cell sample
  47. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH (2018) IF: 1.492
    Propofol attenuates sepsis-induced acute kidney injury by regulating miR-290-5p/CCL-2 signaling pathway

    DOI: 10.1590/1414-431X20187655
    Sample: serum
  48. Journal of Huazhong University of Science and Technology-Medical Sciences (2016) IF: 0.838
    Peroxisome proliferator-activated receptor-γ agonist pioglitazone fails to attenuate renal fibrosis caused by unilateral ureteral obstruction in mice

    DOI: 10.1007/s11596-016-1539-1
    Sample: Tissue homogenate
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