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Cat.No.:E-EL-M1292
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse VEGF-A were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 94.00 | 212.07 | 852.78 | 83.63 | 230.40 | 893.36 |
Standard deviation | 4.75 | 10.50 | 46.05 | 4.25 | 11.45 | 47.44 |
CV (%) | 5.05 | 4.95 | 5.40 | 5.08 | 4.97 | 5.31 |
The recovery of Mouse VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 97-108 | 103 |
EDTA plasma (n=8) | 98-108 | 101 |
Cell culture media (n=8) | 99-109 | 104 |
Samples were spiked with high concentrations of Mouse VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q00731
|
Synonyms | VEGF-A, VEGFA, MVCD1, VPF |
Research Area | Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 33398131 |
Sample: RCS tissues |
DOI: 10.1111/cpr.13316 |
|
PMID: 35869570 |
Sample: skin samples |
DOI: 10.1002/glia.24109 |
|
Sample: primary astrocytes,pericytes |
Sample: corneal tissues |
PMID: 30856539 |
Sample: Serum |
PMID: 33918641 |
Sample: Cell sample,Serum |
PMID: 36334391 |
Sample: RAW264.7 cells |
DOI: 10.1002/jbmr.3388 |
|
Sample: primary chondrocytes |
PMID: 35815765 |
Sample: corneal tissue |
Sample: brain microvascular endothelial cells (BMECs) |
PMID: 34413777 |
Sample: muscle,serum |
PMID: 36253797 |
Sample: serum |
PMID: 35447198 |
Sample: serum |
PMID: 35248678 |
Sample: gastrocnemius muscles |
Sample: Serum,Tissue homogenate |
Sample: Mesenchymal stem cells (MSCs) |
Sample: serum |