Current Position:Home>>Kits>>ELISA Kits>> MS Mouse IL-1ra/IL-1F3(Interleukin 1 Receptor Antagonist) ELISA Kit
Cat.No.:E-MSEL-M0058
Welcome to order from local distributors.
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 15.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Mouse IL-1ra/IL-1F3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1ra/IL-1F3 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-1ra/IL-1F3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-1ra/IL-1F3 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 107.81 | 298.31 | 706.86 | 106.60 | 270.20 | 704.32 |
Standard deviation | 5.46 | 17.78 | 21.84 | 6.12 | 11.65 | 29.58 |
CV (%) | 5.06 | 5.96 | 3.09 | 5.74 | 4.31 | 4.20 |
The recovery of Mouse IL-1ra/IL-1F3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-104 | 97 |
EDTA plasma (n=8) | 92-109 | 99 |
Cell culture media (n=8) | 95-107 | 101 |
Samples were spiked with high concentrations of Mouse IL-1ra/IL-1F3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P25085
|
Synonyms | IL1RN, DIRA, ICIL-1RA, IL-1RN, IL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 |
Research Area | Cancer, Immunology |