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MS Mouse LEP(Leptin) ELISA Kit

Citations(18)Uniprot : P41160

Cat.No.:E-MSEL-M0033
Reactivity: Mouse

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MS Series

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	48T/96T/96
Assay time	3.5h
Detection range	0.63-40 ng/mL
Sensitivity	0.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 25μL
Specificity	This kit recognizes Mouse LEP in samples. No significant cross-reactivity or interference between Mouse LEP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse LEP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse LEP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.13	5.51	15.51	2.27	5.09	16.44
Standard deviation	0.11	0.24	0.79	0.13	0.28	0.61
CV (%)	5.15	4.38	5.12	5.93	5.59	3.73

Recovery

The recovery of Mouse LEP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-108	100
EDTA plasma (n=8)	93-108	101
Cell culture media (n=8)	90-105	96

Linearity

Samples were spiked with high concentrations of Mouse LEP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P41160
Synonyms	LEPD, OB, OBS, Obesity Homolog
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

Citations

FREE RADICAL BIOLOGY AND MEDICINE (2018) IF: 6.02
Procyanidin B2 ameliorates free fatty acids-induced hepatic steatosis through regulating TFEB-mediated lysosomal pathway and redox state

DOI: 10.1016/j.freeradbiomed.2018.08.024

Sample: serum
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES (2021) IF: 6.053
Differential Responses of White Adipose Tissue and Brown Adipose Tissue to Calorie Restriction During Aging

DOI: 10.1093/gerona/glaa070

PMID: 32222773
Sample: serum
ENVIRONMENTAL POLLUTION (2020) IF: 6.793
Effects of incremental endosulfan sulfate exposure and high fat diet on lipid metabolism, glucose homeostasis and gut microbiota in mice

DOI: 10.1016/j.envpol.2020.115697

PMID: 33070067
Sample: Serum
CHEMOSPHERE (2019) IF: 5.108
Neonicotinoid insecticides exposure cause amino acid metabolism disorders, lipid accumulation and oxidative stress in ICR mice

DOI: 10.1016/j.chemosphere.2019.125661

PMID: 31891846
Sample: Serum
Nutrients (2021) IF: 5.719
Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet

DOI: 10.3390/nu13031017

PMID: 33801119
Sample: Serum
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2021) IF: 4.076
Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus

DOI: 10.1080/13813455.2021.1894178

PMID: 33663304
Sample: Serum
Biomolecules (2020) IF: 4.082
Hypothermic Effect of Acute Citral Treatment during LPS-induced Systemic Inflammation in Obese Mice: Reduction of Serum TNF-α and Leptin Levels

DOI: 10.3390/biom10101454

PMID: 33080865
Sample: Serum
Food & Function (2020) IF: 4.171
A multi-targeting strategy to ameliorate high-fat-diet- and fructose-induced (western diet-induced) non-alcoholic fatty liver disease (NAFLD) with supplementation of a mixture of legume ethanol extracts

DOI: 10.1039/D0FO01405B

PMID: 32815965
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues

DOI: 10.3390/ijms21145121

Sample: serum
Journal of Functional Foods (2019) IF: 3.197
The protective effects of Chinese yam polysaccharide against obesity-induced insulin resistance

DOI: 10.1016/j.jff.2019.02.023

Sample: Serum
EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
Pancreastatin inhibitor PSTi8 protects the obesity associated skeletal muscle insulin resistance in diet induced streptozotocin-treated diabetic mice

DOI: 10.1016/j.ejphar.2020.173204

Sample: plasma
TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347
Role of TRPV1/TRPV3 channels in olanzapine-induced metabolic alteration: Possible involvement in hypothalamic energy-sensing, appetite regulation, inflammation and mesolimbic pathway

DOI: 10.1016/j.taap.2020.115124

PMID: 32652086
Sample: Serum
TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585
Modeling of antipsychotic-induced metabolic alterations in mice: An experimental approach precluding psychosis as a predisposing factor

DOI: 10.1016/j.taap.2019.114643

PMID: 31254565
Sample: Serum
LIFE SCIENCES (2020) IF: 3.647
Berberine attenuated olanzapine-induced metabolic alterations in mice: Targeting transient receptor potential vanilloid type 1 and 3 channels

DOI: 10.1016/j.lfs.2020.117442

Sample: serum
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY (2020) IF: 2.056
Virgin Grape Seed Oil Alleviates Insulin Resistance and Energy Metabolism Disorder in Mice Fed a High‐Fat Diet

DOI: 10.1002/ejlt.201900158

Sample: Serum
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
Astragalin, (3-O-glucoside of kaempferol), isolated from Moringa oleifera leaves modulates leptin, adiponectin secretion and inhibits adipogenesis in 3T3-L1 adipocytes

DOI: 10.1080/13813455.2020.1740742

Sample: 3T3-L1 cell
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

DOI: 10.1155/2021/8367802

PMID: 33643424
Sample: Plasma
Physiological Reports (2017)
Is the FVB/N mouse strain truly resistant to diet-induced obesity?

DOI: 10.14814/phy2.13271

Sample: serum

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