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Cat.No.:E-MSEL-M0056
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Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 9.65 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse NGF concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NGF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NGF were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 92.81 | 252.60 | 909.20 | 94.33 | 261.26 | 973.95 |
Standard deviation | 6.04 | 14.75 | 36.19 | 6.09 | 14.89 | 35.55 |
CV (%) | 6.51 | 5.84 | 3.98 | 6.46 | 5.70 | 3.65 |
The recovery of Mouse NGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-106 | 98 |
EDTA plasma (n=8) | 85-100 | 92 |
Cell culture media (n=8) | 93-106 | 99 |
Samples were spiked with high concentrations of Mouse NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P01139
|
Synonyms | Beta-NGF, HSAN5, NGFB, NGF-B |
Research Area | Cancer, Neuroscience |