Ni Focurose 6FF (TED)

    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
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    >
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience
    • Ni-6FF(TED)-Elabscience

      Catalog number:E-CM-AF06

      Size:
      • 25mL
      • 100mL
      • 300mL
      • 500mL
      • 1L
      Qty:
      - +
      Price: $313

      Matrix: Highly cross-linked 6% agarose

      Particle size range: 45-165 µm

      Average particle size: 90 µm

      Pressure: ≤ 0.3 MPa

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Product introduction

      Ni-6FF (TED) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+. The strong binding Ni2+ can be directly used for His-tagged protein expressed by eukaryotic expression system, and it have resistance for higher concentration of reductant and chelating agent. In addition, sample pretreatment is unnecessary. The cleaning and reviving of media are simple, which can be washed directly without nickel removal.  

       

      Advantages

      1.  Quick and easy (one-step purification).

      2.  Tolerate to higher concentration of reductant and chelating agent. His-tagged protein expressed by eukaryotic expression system can be loading without pretreatment, which can protect the protein activity to the utmost.

      3.  There is no need to remove nickel removal can wash with NaOH directly, which greatly shorten the cleaning period.

      4.  Lower Ni2+ abscission than Ni-6FF (IMAC) and Ni-6FF (IDA). No need of repeated revival.

       

      Performance index

      Matrix

      Highly cross-linked 6% agarose

      Particle size range

      45-165 µm

      Average particle size

      90 µm

      Binding capacity

      20~30 mg (His-tagged protein)/mL (media)

      pH stability

      3-12 (working)

      2-14 (washing)

      Chemical stability

      0.01MHCl, 0.01M NaOH (1 week)

      20 mM EDTA, 10 mM DTT, 1M NaOH, 8M Urea, 6M Guanidine hydrochloride (24 hours)

      100 mM EDTA, 0.5M Iminazole (2 hours

      30% Isopropyl alcohol (20 minutes)

      Flow rate

      150-600 cm/h

      (0.3MPa, XK16/40. Column bed height:30 cm)

      Pressure

      0.3 MPa

      Storage buffer

      20% Ethanol

      Storage temperature

      4~8

       


      Application examples

      Example 1:






      Example 2:




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