When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. After extraction and loading for electrophoresis, a DNA ladder of 180-200bp can be found in such DNA samples, which is a specific event during cell apoptosis. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with labeled dUTP, which can be detected with microscopy or flow cytometry.
Elabscience® One-step TUNEL Assay Kit is a highly sensitive, fast and simple method for cell apoptosis detection. The test results can be directly observed through a fluorescence microscope. This kit is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
|Cat.||Products||20 Assays||50 Assays||100 Assays|
|E-CK-A32A||TdT Equilibration Buffer||4 mL||9 mL||9 mL × 2|
|E-CK-A32B||TdT Enzyme||100 μL||250 μL||250 μL × 2|
|E-CK-A32C||Proteinase K (100 ×)||20 μL||50 μL||100 μL|
|E-CK-A323D||Labeling Solution||100 μL × 2||100 μL × 5||100 μL × 10|
|E-CK-A32E||DNase I (2 U/μL)||5 μL||13 μL||25 μL|
|E-CK-A32F||DNase I Buffer (10 ×)||100 μL||250 μL||500 μL|
|E-CK-A32G||PI (25 ×)||100μL||250μL||500μL|
Fixative Buffer (Polyformaldehyde dissolved in PBS with final concentration of 4%) (It is recommended to use Elabscience®E-IR-R113).
Permeablilization Buffer (Triton-100 dissolved in PBS with final concentration of 0.2%) (It is recommended to use Elabscience® E-IR-R122).
Fixative Buffer (Polyformaldehyde dissolved in PBS with final concentration of 4%) (It is recommended to use Elabscience® E-IR-R113).
Store at -20°C for 12 months. Labeling Solution and PI (25 ×) should be stored in the dark.
1.Avoid repeated freezing and thawing of the Label Solution and TdT enzyme, Vortex and mix well before use.
2.After washing the slides with PBS, please wipe dry the liquid around the tissue on the slides with absorbent paper.
3.Keep the sample moist during the experiment to prevent the failure of the experiment caused by dry slices.
4.This kit is for research use only. For your safety and health, please wear lab clothes and gloves. Instructions should be followed strictly, changes of operation may result in unreliable results.
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