Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Mouse FcεRIα Antibody[MAR-1]
Cat.No.:E-AB-F1188UD
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | High affinity immunoglobulin epsilon receptor subunit alpha,Fc-epsilon RI-alpha,FcERI,Fcer1a |
Swissprot | P20489 |
References |
1. Arinobu Y, et al. 2005. P. Natl. Acad. Sci. USA 102:18105.
2. Yamaguchi M, et al. 2001. Int. Immunol. 13:843. |
Form | Liquid |
Clone No. | MAR-1(See Other Available Formats) |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Isotype Control | PE Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09853D] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
FcεRIα is a transmembrane protein belonging to the Ig superfamily. FcεRIα forms a tetrameric complex with one β and two γ-subunits. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions. It is abundantly expressed on mast and basophils and up-regulated by the presence of IgE. Following stimulation via FcεRIα, mast cells and basophils release bioactive chemical mediators such as histamine, resulting in the initiation of allergic reactions. Cross linking of the high-affinity receptor for IgE on tissue mast cells triggers immediate hypersensitivity with local symptoms. The MAR-1 monoclonal antibody reacts with the FcεRIα subunit. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Mouse FcεRIα Antibody[MAR-1] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse FcεRIα Antibody[MAR-1] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse FcεRIα Antibody[MAR-1] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Mouse FcεRIα Antibody[MAR-1] | 25μg, 100μg | Check More |
APC Anti-Mouse FcεRIα Antibody[MAR-1] | 25μg, 100μg | Check More |