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PerCP/Cyanine5.5 Anti-Human CD22 Antibody[BU59]

Uniprot : P20273
  • Cat.No.:E-AB-F1047J

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1047J

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  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $102
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Antigen Details

Alternate Names B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2
Swissprot P20273
References
1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466.

Product Details

Form Liquid
Clone No. BU59
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792J]
Reactivity Human
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP/Cyanine5.5
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Human CD22 Antibody[BU59] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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