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PerCP/Cyanine5.5 Anti-Human CD34 Antibody[581]

Uniprot : P28906
  • Cat.No.:E-AB-F1143J

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1143J

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $120
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Antigen Details

Alternate Names Gp105-120, My10
Swissprot P28906
References
1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Product Details

Form Liquid
Clone No. 581
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792J]
Reactivity Human
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP/Cyanine5.5
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD34 Antibody[581] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD34 Antibody[581] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD34 Antibody[581] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD34 Antibody[581] 20Tests, 100Tests, 100Tests×2 Check More

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