Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Rat CD3 Antibody[G4.18]
Cat.No.:E-AB-F1228UJ
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | T-cell surface glycoprotein CD3 δ, γ, ε, and ζ chains,CD3 Complex, T3,CD3 |
Swissprot | P19377,Q64159,D4A5M2 |
References |
1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. |
Form | Liquid |
Clone No. | G4.18(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG3, κ |
Isotype Control | PerCP/Cyanine5.5 Mouse IgG3, κ Isotype Control[A112-3] [Product E-AB-F09753J] |
Reactivity | Rat |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PerCP/Cyanine5.5 |
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Elab Fluor® 594 Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® Red 780 Anti-Rat CD3 Antibody[G4.18] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Rat CD3 Antibody[G4.18] | 25μg, 100μg | Check More |
PE Anti-Rat CD3 Antibody[G4.18] | 25μg, 100μg | Check More |
APC Anti-Rat CD3 Antibody[G4.18] | 25μg, 100μg | Check More |
PE/Elab Fluor® 594 Anti-Rat CD3 Antibody[G4.18] | 25μg, 100μg | Check More |
Elab Fluor® Red 780 Anti-Rat CD3 Antibody[G4.18] | 25μg, 100μg | Check More |
DOI: 10.1155/2022/2408598 |
|
Sample: immune cell |