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QuicKey Human MAU(Microalbuminuria) ELISA Kit

Uniprot : P02768
  • Cat.No.:E-TSEL-H0005

  • Reactivity: Human

To Purchase E-TSEL-H0005

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $390
Qty:

Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.30 ng/mL
Sample type &Sample volume serum, plasma; 50μL
Specificity This kit recognizes Human MAU in samples. No significant or interference between Human MAU and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human MAU concentrations in serum, plasma.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MAU. Samples (or Standards) and biotinylated detection antibody specific for Human MAU are added to the micro ELISA plate wells. Human MAU would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human MAU. You can calculate the concentration of Human MAU in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Sample Values

sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MAU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MAU were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.14 12.39 49.50 4.74 11.35 40.46
Standard deviation 0.22 0.56 2.66 0.24 0.56 2.04
CV (%) 5.31 4.52 5.37 5.06 4.93 5.04

Recovery

The recovery of Human MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Urine(n=8) 97-104 101

Linearity

Samples were spiked with high concentrations of Human MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

sandwich-Ab-ELISA-Elabscience

Target Information

Background

Microalbuminuria is a subtle increase in the urinary excretion of the protein albumin that cannot be detected by a conventional assay. In diabetes, microalbuminuria is an early sign of diabetic kidney disease. Specifically, the excretion of greater than 30 mg and less than 300 mg a day of albumin in the urine. The normal urinary albumin is less than 30 mg per 24 hours and 300 mg or more of urinary albumin per day is considered gross albuminuria. The phenomenon of albuminuria has been recognized for more than 200 years, and its association with kidney disease dates to the epochal insights of Richard Bright in 1827[1]. Microalbuminuria is caused by glomerular capillary injury and so may be a marker for diffuse endothelial dysfunction. According to Steno hypothesis, albuminuria might reflect a general vascular dysfunction and leakage of albumin and other plasma macromolecules such as low density lipoproteins into the vessel wall that may lead to inflammatory responses and in turn start the atherosclerotic process [2].
1. Glassock R J. Prevention of Microalbuminuria in Type 2 Diabetes: Millimeters or Milligrams? [J]. Journal of the American Society of Nephrology Jasn, 2006, 17(12):3276.
2. Deckert T, Feldtrasmussen B, Borchjohnsen K, et al. Albuminuria reflects widespread vascular damage [J]. Diabetologia, 1989, 32(4):219.
Database LinksSwissProt: P02768
Entrez Gene: 213
SynonymsUMA, mAlb
Research AreaImmunology,Metabolism

Assay Procedures

step01

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C

step02

2. Aspirate and wash the plate for 3 times

step03

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

step04

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

step05

5. Add 50μL Stop Solution

step06

6. Read the plate at 450nm immediately. Calculation of the results

Citations

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